Sensitivity to endotoxin in rabbits is increased after hemorrhagic shock

William Mileski, R. K. Winn, J. M. Harlan, C. L. Rice

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The immunoinflammatory response following trauma and hemorrhage may predispose to the development of sepsis and multiple-organ failure syndrome. Cardiac output (CO), arterial pressure, arterial PO2, and pulmonary permeability index were measured. We examined the sensitivity of rabbits to infusions of lipopolysaccharide (LPS) after hemorrhagic shock. Shock was produced by reducing CO to 40% of baseline for 90 min, followed by resuscitation with shed blood and then with lactated Ringer solution to maintain CO near baseline. Animals were assigned to three groups: 1) hemorrhagic shock only, 2) LPS only, and 3) hemorrhagic shock + LPS. Groups 1 and 3 were subjected to hemorrhagic shock on day 1. Escherichia coli LPS was infused (1.0 μg/kg iv) into groups 2 and 3 on day 2. Fluid resuscitation with lactated Ringer solution was continued in an effort to maintain CO at baseline. Five hours after LPS infusion, 125I-albumin was injected intravenously, and rabbits were killed 1 h later for measurement of pulmonary permeability index. LPS infusion after shock (group 3) caused significant decreases in CO, arterial pressure, and PO2 and an increase in pulmonary permeability. These changes were not seen in the groups 1 and 2. We conclude that hemorrhagic shock and resuscitation result in a proinflammatory state, leading to increased sensitivity to subsequent exposure to LPS.

Original languageEnglish
Pages (from-to)1146-1149
Number of pages4
JournalJournal of Applied Physiology
Volume73
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Endotoxins
Lipopolysaccharides
Rabbits
Cardiac Output
Resuscitation
Permeability
Lung
Shock
Arterial Pressure
Multiple Organ Failure
Albumins
Sepsis
Hemorrhage
Escherichia coli
Wounds and Injuries

Keywords

  • hemorrhagic shock
  • lung permeability
  • multiple-organ failure
  • sepsis

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Sensitivity to endotoxin in rabbits is increased after hemorrhagic shock. / Mileski, William; Winn, R. K.; Harlan, J. M.; Rice, C. L.

In: Journal of Applied Physiology, Vol. 73, No. 3, 1992, p. 1146-1149.

Research output: Contribution to journalArticle

Mileski, W, Winn, RK, Harlan, JM & Rice, CL 1992, 'Sensitivity to endotoxin in rabbits is increased after hemorrhagic shock', Journal of Applied Physiology, vol. 73, no. 3, pp. 1146-1149.
Mileski, William ; Winn, R. K. ; Harlan, J. M. ; Rice, C. L. / Sensitivity to endotoxin in rabbits is increased after hemorrhagic shock. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 3. pp. 1146-1149.
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