Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism

R. A. Barke, S. Roy, R. B. Chapin, R. Charboneau, P. S. Brady, L. J. Brady, Courtney Townsend, C. Hauser, R. D. Beauchamp

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. The immediate-early gene c-fos has been implicated in transcriptional regulation after sepsis. We test the hypothesis that sepsis- induced central nervous system release of interleukin (IL)-6 regulates hepatic c-fos gene expression. Methods. Using a stereotaxically placed intracerebral-ventricular (ICV) catheter in rats with and without hypophysectomy, we measured hepatic c-fos protein accumulation after treatment with either IL-6 or vehicle control. Using a rat cecal ligation and puncture (CLP) model, we studied the following groups: (1) sham-CLP, (2), (3) hypophysectomized sham-CLP, and (4) hypophysectomized CLP and measured hepatic c-fos mRNA. Results. ICV IL-6 treatment increased hepatic c-fos protein in the IL-6-treated group compared with the vehicle-treated group, and hypophysectomy inhibited the ICV IL-6-mediated increase in c-fos protein. After peritoneal sepsis, CLP increased hepatic c-fos messenger RNA compared to either the sham-CLP or the hypophysectomized sham-CLP group, and hypophysectomy before CLP inhibited hepatic c-fos mRNA compared with the CLP group. Conclusions. ICV IL-6 results in an increase in hepatic fos protein that is mediated through a hypothalamic-hypophyseal mechanism. Peritoneal sepsis results in an increase in hepatic c-fos gene expression that may be, in part, mediated by central nervous system release of IL-6 through a hypothalamic-hypophyseal mechanism.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalSurgery
Volume116
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Immediate-Early Genes
Punctures
Ligation
Interleukin-6
Sepsis
Liver
Proto-Oncogene Proteins c-fos
Hypophysectomy
fos Genes
Messenger RNA
Central Nervous System
Gene Expression
Catheters

ASJC Scopus subject areas

  • Surgery

Cite this

Barke, R. A., Roy, S., Chapin, R. B., Charboneau, R., Brady, P. S., Brady, L. J., ... Beauchamp, R. D. (1994). Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism. Surgery, 116(2), 141-149.

Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism. / Barke, R. A.; Roy, S.; Chapin, R. B.; Charboneau, R.; Brady, P. S.; Brady, L. J.; Townsend, Courtney; Hauser, C.; Beauchamp, R. D.

In: Surgery, Vol. 116, No. 2, 1994, p. 141-149.

Research output: Contribution to journalArticle

Barke, RA, Roy, S, Chapin, RB, Charboneau, R, Brady, PS, Brady, LJ, Townsend, C, Hauser, C & Beauchamp, RD 1994, 'Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism', Surgery, vol. 116, no. 2, pp. 141-149.
Barke RA, Roy S, Chapin RB, Charboneau R, Brady PS, Brady LJ et al. Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism. Surgery. 1994;116(2):141-149.
Barke, R. A. ; Roy, S. ; Chapin, R. B. ; Charboneau, R. ; Brady, P. S. ; Brady, L. J. ; Townsend, Courtney ; Hauser, C. ; Beauchamp, R. D. / Sepsis-induced release of interleukin-6 may activate the immediate-early gene program through a hypothalamic-hypophyseal mechanism. In: Surgery. 1994 ; Vol. 116, No. 2. pp. 141-149.
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abstract = "Background. The immediate-early gene c-fos has been implicated in transcriptional regulation after sepsis. We test the hypothesis that sepsis- induced central nervous system release of interleukin (IL)-6 regulates hepatic c-fos gene expression. Methods. Using a stereotaxically placed intracerebral-ventricular (ICV) catheter in rats with and without hypophysectomy, we measured hepatic c-fos protein accumulation after treatment with either IL-6 or vehicle control. Using a rat cecal ligation and puncture (CLP) model, we studied the following groups: (1) sham-CLP, (2), (3) hypophysectomized sham-CLP, and (4) hypophysectomized CLP and measured hepatic c-fos mRNA. Results. ICV IL-6 treatment increased hepatic c-fos protein in the IL-6-treated group compared with the vehicle-treated group, and hypophysectomy inhibited the ICV IL-6-mediated increase in c-fos protein. After peritoneal sepsis, CLP increased hepatic c-fos messenger RNA compared to either the sham-CLP or the hypophysectomized sham-CLP group, and hypophysectomy before CLP inhibited hepatic c-fos mRNA compared with the CLP group. Conclusions. ICV IL-6 results in an increase in hepatic fos protein that is mediated through a hypothalamic-hypophyseal mechanism. Peritoneal sepsis results in an increase in hepatic c-fos gene expression that may be, in part, mediated by central nervous system release of IL-6 through a hypothalamic-hypophyseal mechanism.",
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AU - Barke, R. A.

AU - Roy, S.

AU - Chapin, R. B.

AU - Charboneau, R.

AU - Brady, P. S.

AU - Brady, L. J.

AU - Townsend, Courtney

AU - Hauser, C.

AU - Beauchamp, R. D.

PY - 1994

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N2 - Background. The immediate-early gene c-fos has been implicated in transcriptional regulation after sepsis. We test the hypothesis that sepsis- induced central nervous system release of interleukin (IL)-6 regulates hepatic c-fos gene expression. Methods. Using a stereotaxically placed intracerebral-ventricular (ICV) catheter in rats with and without hypophysectomy, we measured hepatic c-fos protein accumulation after treatment with either IL-6 or vehicle control. Using a rat cecal ligation and puncture (CLP) model, we studied the following groups: (1) sham-CLP, (2), (3) hypophysectomized sham-CLP, and (4) hypophysectomized CLP and measured hepatic c-fos mRNA. Results. ICV IL-6 treatment increased hepatic c-fos protein in the IL-6-treated group compared with the vehicle-treated group, and hypophysectomy inhibited the ICV IL-6-mediated increase in c-fos protein. After peritoneal sepsis, CLP increased hepatic c-fos messenger RNA compared to either the sham-CLP or the hypophysectomized sham-CLP group, and hypophysectomy before CLP inhibited hepatic c-fos mRNA compared with the CLP group. Conclusions. ICV IL-6 results in an increase in hepatic fos protein that is mediated through a hypothalamic-hypophyseal mechanism. Peritoneal sepsis results in an increase in hepatic c-fos gene expression that may be, in part, mediated by central nervous system release of IL-6 through a hypothalamic-hypophyseal mechanism.

AB - Background. The immediate-early gene c-fos has been implicated in transcriptional regulation after sepsis. We test the hypothesis that sepsis- induced central nervous system release of interleukin (IL)-6 regulates hepatic c-fos gene expression. Methods. Using a stereotaxically placed intracerebral-ventricular (ICV) catheter in rats with and without hypophysectomy, we measured hepatic c-fos protein accumulation after treatment with either IL-6 or vehicle control. Using a rat cecal ligation and puncture (CLP) model, we studied the following groups: (1) sham-CLP, (2), (3) hypophysectomized sham-CLP, and (4) hypophysectomized CLP and measured hepatic c-fos mRNA. Results. ICV IL-6 treatment increased hepatic c-fos protein in the IL-6-treated group compared with the vehicle-treated group, and hypophysectomy inhibited the ICV IL-6-mediated increase in c-fos protein. After peritoneal sepsis, CLP increased hepatic c-fos messenger RNA compared to either the sham-CLP or the hypophysectomized sham-CLP group, and hypophysectomy before CLP inhibited hepatic c-fos mRNA compared with the CLP group. Conclusions. ICV IL-6 results in an increase in hepatic fos protein that is mediated through a hypothalamic-hypophyseal mechanism. Peritoneal sepsis results in an increase in hepatic c-fos gene expression that may be, in part, mediated by central nervous system release of IL-6 through a hypothalamic-hypophyseal mechanism.

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