Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV

Abdellah Akil; Juan Peng; Mohyeddine Omrane; Claire Gondeau; Christophe Desterke; Mickaël Marin; Hélène Tronchère; Cyntia Taveneau; Sokhavuth Sar; Philippe Briolotti; Soumaya Benjelloun; Abdelaziz Benjouad; Patrick Maurel; Valérie Thiers; Stéphane Bressanelli; Didier Samuel; Christian Bréchot; Ama Gassama-Diagne

doi:10.1038/ncomms12203

Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV

Abdellah Akil
, Juan Peng
, Mohyeddine Omrane
, Claire Gondeau
, Christophe Desterke
, Mickaël Marin
, Hélène Tronchère
, Cyntia Taveneau
, Sokhavuth Sar
, Philippe Briolotti
, Soumaya Benjelloun
, Abdelaziz Benjouad
, Patrick Maurel
, Valérie Thiers
, Stéphane Bressanelli
, Didier Samuel
, Christian Bréchot
, Ama Gassama-Diagne

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway.

Original language	English (US)
Article number	12203
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12203
State	Published - Jul 15 2016
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General
General Physics and Astronomy

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10.1038/ncomms12203

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Akil, A., Peng, J., Omrane, M., Gondeau, C., Desterke, C., Marin, M., Tronchère, H., Taveneau, C., Sar, S., Briolotti, P., Benjelloun, S., Benjouad, A., Maurel, P., Thiers, V., Bressanelli, S., Samuel, D., Bréchot, C., & Gassama-Diagne, A. (2016). Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nature communications, 7, Article 12203. https://doi.org/10.1038/ncomms12203

Akil, A, Peng, J, Omrane, M, Gondeau, C, Desterke, C, Marin, M, Tronchère, H, Taveneau, C, Sar, S, Briolotti, P, Benjelloun, S, Benjouad, A, Maurel, P, Thiers, V, Bressanelli, S, Samuel, D, Bréchot, C & Gassama-Diagne, A 2016, 'Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV', Nature communications, vol. 7, 12203. https://doi.org/10.1038/ncomms12203

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title = "Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV",

abstract = "The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway.",

author = "Abdellah Akil and Juan Peng and Mohyeddine Omrane and Claire Gondeau and Christophe Desterke and Micka{\"e}l Marin and H{\'e}l{\`e}ne Tronch{\`e}re and Cyntia Taveneau and Sokhavuth Sar and Philippe Briolotti and Soumaya Benjelloun and Abdelaziz Benjouad and Patrick Maurel and Val{\'e}rie Thiers and St{\'e}phane Bressanelli and Didier Samuel and Christian Br{\'e}chot and Ama Gassama-Diagne",

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AU - Akil, Abdellah

AU - Peng, Juan

AU - Omrane, Mohyeddine

AU - Gondeau, Claire

AU - Desterke, Christophe

AU - Marin, Mickaël

AU - Tronchère, Hélène

AU - Taveneau, Cyntia

AU - Sar, Sokhavuth

AU - Briolotti, Philippe

AU - Benjelloun, Soumaya

AU - Benjouad, Abdelaziz

AU - Maurel, Patrick

AU - Thiers, Valérie

AU - Bressanelli, Stéphane

AU - Samuel, Didier

AU - Bréchot, Christian

AU - Gassama-Diagne, Ama

PY - 2016/7/15

Y1 - 2016/7/15

N2 - The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway.

AB - The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway.

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DO - 10.1038/ncomms12203

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JO - Nature communications

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Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV

Abstract

ASJC Scopus subject areas

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