Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli

Wen Bing Wang, Ping Ji, Jun Wu, Jia Lu He, Xiang Fu Wu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576. 13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD1.

Original languageEnglish (US)
Pages (from-to)407-408
Number of pages2
JournalActa Biochimica et Biophysica Sinica
Volume31
Issue number4
StatePublished - 1999
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Bombyx
Viruses
Escherichia coli
Sequence Analysis
Genes
Oxidative stress
DNA sequences
DNA Sequence Analysis
Superoxides
Superoxide Dismutase
Oxidative Stress
Nucleotides
Amino Acids
Polymerase Chain Reaction

Keywords

  • BmNPV
  • Expression
  • Sequence analysis
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Sequence analysis of SOD gene of Bombyx mori nuclear polyhedrosis virus and it's expression in E. coli. / Wang, Wen Bing; Ji, Ping; Wu, Jun; He, Jia Lu; Wu, Xiang Fu.

In: Acta Biochimica et Biophysica Sinica, Vol. 31, No. 4, 1999, p. 407-408.

Research output: Contribution to journalArticle

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AU - He, Jia Lu

AU - Wu, Xiang Fu

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