Sequence analysis of the small RNA segment of guinea pig-passaged Pichinde virus variants

Lihong Zhang, Kathleen Marriott, Judith Aronson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The established animal model for Lassa fever is based on the new world arenavirus Pichinde (PIC). Natural isolates of PIC virus are attenuated in guinea pigs, but serial guinea pig passage renders them extremely virulent in that host. We have compared the nucleotide sequences of the small RNA segments of two attenuated, lowpassage variants of the PIC virus Munchique strain (CoAn 4763) and two virulent, high-passage derivatives. Missense mutations in the glycoprotein precursor (GPC) gene at codons GPC-119, GPC- 140, and GPC-164 and the nucleoprotein gene (NP) codons NP-35 and NP-374 were most closely associated with virulence. Codon GPC-140 is predicted to represent a region of peak hydrophilicity of the glycoprotein 1 (GP1); it is conceivable that mutations at this site could influence virulence by altering B cell epitopes or virus attachment protein conformation.

Original languageEnglish (US)
Pages (from-to)220-225
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume61
Issue number2
StatePublished - Aug 1999

Fingerprint

Pichinde virus
RNA Sequence Analysis
Nucleoproteins
Glycoproteins
Guinea Pigs
Codon
Genes
Virulence
New World Arenaviruses
Lassa Fever
B-Lymphocyte Epitopes
Cercopithecine Herpesvirus 1
Virus Attachment
Protein Conformation
Missense Mutation
Hydrophobic and Hydrophilic Interactions
Animal Models
RNA
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Sequence analysis of the small RNA segment of guinea pig-passaged Pichinde virus variants. / Zhang, Lihong; Marriott, Kathleen; Aronson, Judith.

In: American Journal of Tropical Medicine and Hygiene, Vol. 61, No. 2, 08.1999, p. 220-225.

Research output: Contribution to journalArticle

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