Sequence-dependent dynamics of DNA

David P. Millar, Lawrence Sowers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

The sequence-dependent high-frequency motion of a fluorescent base in DNA has been studied by picosecond time-resolved fluorescence depolarization measurements on a series of defined-sequence synthetic oligonucleotides of the type d[GCCXCCG] · d[CGG(AP)GGC], where X = T, A, G or C and AP is the fluorescent adenine analogue 2-aminopurine. The fluorescence anisotropy contained a rapidly decaying component that was due to restricted internal rotation of AP and a slowly decaying component due to overall tumbling. The tumbling correlation time varied with X in a manner that reflected the association state of the oligonucleotide strands. The internal motion dynamics of AP depended on the nature of the base-pairing interactions between AP and X.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
Place of PublicationBellingham, WA, United States
PublisherPubl by Int Soc for Optical Engineering
Pages656-662
Number of pages7
Volume1204 pt 2
ISBN (Print)0819402451
StatePublished - 1990
Externally publishedYes
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period1/15/901/17/90

Fingerprint

Barreling
oligonucleotides
Oligonucleotides
DNA
deoxyribonucleic acid
Fluorescence
fluorescence
adenines
Depolarization
depolarization
strands
Anisotropy
Association reactions
analogs
anisotropy
interactions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Millar, D. P., & Sowers, L. (1990). Sequence-dependent dynamics of DNA. In J. F. Lakowicz (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1204 pt 2, pp. 656-662). Bellingham, WA, United States: Publ by Int Soc for Optical Engineering.

Sequence-dependent dynamics of DNA. / Millar, David P.; Sowers, Lawrence.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Joseph F. Lakowicz. Vol. 1204 pt 2 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. p. 656-662.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Millar, DP & Sowers, L 1990, Sequence-dependent dynamics of DNA. in JF Lakowicz (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1204 pt 2, Publ by Int Soc for Optical Engineering, Bellingham, WA, United States, pp. 656-662, Time-Resolved Laser Spectroscopy in Biochemistry II, Los Angeles, CA, USA, 1/15/90.
Millar DP, Sowers L. Sequence-dependent dynamics of DNA. In Lakowicz JF, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1204 pt 2. Bellingham, WA, United States: Publ by Int Soc for Optical Engineering. 1990. p. 656-662
Millar, David P. ; Sowers, Lawrence. / Sequence-dependent dynamics of DNA. Proceedings of SPIE - The International Society for Optical Engineering. editor / Joseph F. Lakowicz. Vol. 1204 pt 2 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. pp. 656-662
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