Sequence-dependent dynamics of DNA

David P. Millar, Larry C. Sowers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations


The sequence-dependent high-frequency motion of a fluorescent base in DNA has been studied by picosecond time-resolved fluorescence depolarization measurements on a series of defined-sequence synthetic oligonucleotides of the type d[GCCXCCG] · d[CGG(AP)GGC], where X = T, A, G or C and AP is the fluorescent adenine analogue 2-aminopurine. The fluorescence anisotropy contained a rapidly decaying component that was due to restricted internal rotation of AP and a slowly decaying component due to overall tumbling. The tumbling correlation time varied with X in a manner that reflected the association state of the oligonucleotide strands. The internal motion dynamics of AP depended on the nature of the base-pairing interactions between AP and X.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
PublisherPubl by Int Soc for Optical Engineering
Number of pages7
ISBN (Print)0819402451
StatePublished - Dec 1 1990
Externally publishedYes
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1204 pt 2
ISSN (Print)0277-786X


OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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