Sequence-Dependent Solvation Dynamics of Minor-Groove Bound Ligand Inside Duplex-DNA

Sachin Dev Verma, Nibedita Pal, Moirangthem Kiran Singh, Sobhan Sen

Research output: Contribution to journalArticlepeer-review

Abstract

Ligand binding to minor-grooves of DNA depends on DNA-base sequence near its binding-site. However, it is not known how base-sequences affect the local solvation of ligand inside minor-grooves of DNA. Here we present a comprehensive study on sequence-dependent solvation dynamics of ligand inside duplex-DNA by measuring the static and dynamic fluorescence Stokes shifts of a popular groove-binder, DAPI, inside DNA minor-grooves created by four different sequences; d(5′-CGCGAATTCGCG-3′)2, d(5′-CGCGTTAACGCG-3′)2, d(5′-CGCGCAATTGCGCG-3′)2, and d(5′-CGCGCTTAAGCGCG-3′)2, having different sequences near DAPI-binding site. Fluorescence up-conversion and time-correlated single photon counting techniques are employed to capture the dynamic Stokes shifts of DAPI over five decades in time from 100 fs to 10 ns. We show that the ligands sense different static and dynamic solvation inside minor-grooves created by different sequences: Only subtle change in the dynamics is seen in DNA containing -AATTG-, -TTAAG-, and -AATTC- sequences, which show power-law relaxation in initial time-decades, followed by biexponential decay in nanosecond time-scales. However, changing a single base (and the complementary base) near ligand-binding site from -TTAAG- to -TTAAC- drastically induces the dynamics to follow a single power-law relaxation over the entire five decades. The observed variation of dynamics possibly relate to the local DNA motions, coupled to the hydration dynamics near the ligand-binding site. (Graph Presented).

Original languageEnglish (US)
Pages (from-to)11019-11029
Number of pages11
JournalJournal of Physical Chemistry B
Volume119
Issue number34
DOIs
StatePublished - Aug 27 2015
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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