Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses

Catherine H. Schein, Mengyi Ye, Aniko V. Paul, M. Steven Oberste, Nora Chapman, Gerbrand J. van der Heden van Noort, Dmitri V. Filippov, Kyung Choi

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Enteroviruses (EV) uridylylate a peptide, VPg, as the first step in their replication. VPgpUpU, found free in infected cells, serves as the primer for RNA elongation. The abilities of four polymerases (3D<sup>pol</sup>), from EV-species A-C, to uridylylate VPgs that varied by up to 60% of their residues were compared. Each 3D<sup>pol</sup> was able to uridylylate all five VPgs using polyA RNA as template, while showing specificity for its own genome encoded peptide. All 3D<sup>pol</sup> uridylylated a consensus VPg representing the physical chemical properties of 31 different VPgs. Thus the residues required for uridylylation and the enzymatic mechanism must be similar in diverse EV. As VPg-binding sites differ in co-crystal structures, the reaction is probably done by a second 3D<sup>pol</sup> molecule. The conservation of polymerase residues whose mutation reduces uridylylation but not RNA elongation is compared.

    Original languageEnglish (US)
    Pages (from-to)80-85
    Number of pages6
    JournalVirology
    Volume484
    DOIs
    StatePublished - Oct 1 2015

    Fingerprint

    Enterovirus
    Genome
    Peptides
    RNA
    Binding Sites
    Mutation

    Keywords

    • Coxsackie virus
    • EV-71
    • Metal ion dependent phosphotransfer
    • Nucleotide transfer reaction
    • PCP-consensus sequence
    • Peptide priming
    • Poliovirus
    • RNA polymerase

    ASJC Scopus subject areas

    • Virology

    Cite this

    Schein, C. H., Ye, M., Paul, A. V., Oberste, M. S., Chapman, N., van der Heden van Noort, G. J., ... Choi, K. (2015). Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses. Virology, 484, 80-85. https://doi.org/10.1016/j.virol.2015.05.016

    Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses. / Schein, Catherine H.; Ye, Mengyi; Paul, Aniko V.; Oberste, M. Steven; Chapman, Nora; van der Heden van Noort, Gerbrand J.; Filippov, Dmitri V.; Choi, Kyung.

    In: Virology, Vol. 484, 01.10.2015, p. 80-85.

    Research output: Contribution to journalArticle

    Schein, CH, Ye, M, Paul, AV, Oberste, MS, Chapman, N, van der Heden van Noort, GJ, Filippov, DV & Choi, K 2015, 'Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses', Virology, vol. 484, pp. 80-85. https://doi.org/10.1016/j.virol.2015.05.016
    Schein CH, Ye M, Paul AV, Oberste MS, Chapman N, van der Heden van Noort GJ et al. Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses. Virology. 2015 Oct 1;484:80-85. https://doi.org/10.1016/j.virol.2015.05.016
    Schein, Catherine H. ; Ye, Mengyi ; Paul, Aniko V. ; Oberste, M. Steven ; Chapman, Nora ; van der Heden van Noort, Gerbrand J. ; Filippov, Dmitri V. ; Choi, Kyung. / Sequence specificity for uridylylation of the viral peptide linked to the genome (VPg) of enteroviruses. In: Virology. 2015 ; Vol. 484. pp. 80-85.
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