Sero-characterization of lipopolysaccharide from Burkholderia thailandensis

Omar Qazi; Joann L. Prior; Barbara M. Judy; Gregory C. Whitlock; G. Barrie Kitto; Alfredo G. Torres; D. Mark Estes; Katherine A. Brown

doi:10.1016/S0035-9203(08)70016-6

Sero-characterization of lipopolysaccharide from Burkholderia thailandensis

Omar Qazi, Joann L. Prior, Barbara M. Judy, Gregory C. Whitlock, G. Barrie Kitto, Alfredo G. Torres, D. Mark Estes, Katherine A. Brown

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Summary: We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.

Original language	English (US)
Pages (from-to)	S58-S60
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	102
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/S0035-9203(08)70016-6
State	Published - Dec 2008
Externally published	Yes

Keywords

Burkholderia
Immune sera
Immunogenic
Lipopolysaccharide
SDS-PAGE
Virulence

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0035-9203(08)70016-6

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title = "Sero-characterization of lipopolysaccharide from Burkholderia thailandensis",

abstract = "Summary: We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.",

keywords = "Burkholderia, Immune sera, Immunogenic, Lipopolysaccharide, SDS-PAGE, Virulence",

author = "Omar Qazi and Prior, {Joann L.} and Judy, {Barbara M.} and Whitlock, {Gregory C.} and Kitto, {G. Barrie} and Torres, {Alfredo G.} and Estes, {D. Mark} and Brown, {Katherine A.}",

note = "Funding Information: Funding: This study was supported by the Western Research Center of Excellence (NIH/NIAID grant U54 AI 057156), NIH/NIAID grant N01-AI-30065, the Welch Foundation (TI-3D grant from the University of Texas at Austin), and the Defence Science and Technology Laboratory, Porton Down, UK. ",

year = "2008",

month = dec,

doi = "10.1016/S0035-9203(08)70016-6",

language = "English (US)",

volume = "102",

pages = "S58--S60",

journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

issn = "0035-9203",

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number = "SUPPL. 1",

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TY - JOUR

T1 - Sero-characterization of lipopolysaccharide from Burkholderia thailandensis

AU - Qazi, Omar

AU - Prior, Joann L.

AU - Judy, Barbara M.

AU - Whitlock, Gregory C.

AU - Kitto, G. Barrie

AU - Torres, Alfredo G.

AU - Estes, D. Mark

AU - Brown, Katherine A.

N1 - Funding Information: Funding: This study was supported by the Western Research Center of Excellence (NIH/NIAID grant U54 AI 057156), NIH/NIAID grant N01-AI-30065, the Welch Foundation (TI-3D grant from the University of Texas at Austin), and the Defence Science and Technology Laboratory, Porton Down, UK.

PY - 2008/12

Y1 - 2008/12

N2 - Summary: We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.

AB - Summary: We report the successful purification of lipopolysaccharide (LPS) from Burkholderia thailandensis, a Gram-negative bacterium, closely related to the highly pathogenic organisms B. pseudomallei and B. mallei. Burkholderia thailandensis LPS is shown to cross-react with rabbit and mouse sera obtained from inoculation with B. pseudomallei or B. mallei, respectively. These data suggest that B. thailandensis LPS shares similar structural features with LPS molecules from highly pathogenic Burkholderia species. This information may prove useful in ongoing efforts to develop novel vaccines and/or diagnostic reagents.

KW - Burkholderia

KW - Immune sera

KW - Immunogenic

KW - Lipopolysaccharide

KW - SDS-PAGE

KW - Virulence

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DO - 10.1016/S0035-9203(08)70016-6

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AN - SCOPUS:58049144526

SN - 0035-9203

VL - 102

SP - S58-S60

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - SUPPL. 1

ER -

Sero-characterization of lipopolysaccharide from Burkholderia thailandensis

Abstract

Keywords

ASJC Scopus subject areas

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