Serodiagnosis of the acquired immune deficiency syndrome by enzyme-linked immunosorbent assay compared to cellular immunologic parameters in African AIDS patients and controls

H. L. Francis, J. Mann, R. L. Colebunders, L. Ndongala, D. Mavunza-Mpaka, K. Bila, J. Curran, P. Piot, T. C. Quinn

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA), absolute numbers of T-helper cells, and T-helper:T-suppressor cell ratios were compared in asymptomatic controls and IgG Western blot-confirmed patients with the acquired immune deficiency syndrome (AIDS) in Kinshasa, Zaire, between August 1984 and May 1985. Two hundred sixteen (97.7%) of 221 IgG Western blot-positive AIDS patients and 4 of 97 (4%) controls were ELISA-positive, 3 of whom were Western blot-positive. The sensitivity and specificity of the ELISA was 97.7% and 99.0%, respectively, compared to Western blot results. Detection of the human immune deficiency virus using absolute number of T-helper cells (<400 cells/mm3) was as sensitive (98.2%), but less specific (90.7%). A T-helper:T-suppressor ratio of <0.9, had a sensitivity of 97.3%, and specificity of 94.8%. The ELISA test had the highest predictive value and greatest utility in an African clinical setting for detecting HIV infected patients where a wide range of other immunocompromising diseases are seen.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume38
Issue number3
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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