Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad

N. N. Thompson, A. J. Auguste, A. P A Travassos da Rosa, C. V F Carrington, B. J. Blitvich, D. D. Chadee, R. B. Tesh, Scott Weaver, A. A. Adesiyun

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P < 0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalZoonoses and Public Health
Volume62
Issue number1
DOIs
StatePublished - Feb 1 2015

Fingerprint

Flaviviridae
Trinidad and Tobago
Alphavirus
Flavivirus
serological surveys
Chiroptera
tetrachloroisophthalonitrile
Rio Bravo virus
Viruses
Venezuelan Equine Encephalitis Viruses
Venezuelan equine encephalitis virus
antibodies
Antibodies
viruses
Eastern equine encephalitis virus
West Nile virus
hemagglutination inhibition test
Hemagglutination
seroprevalence
St. Louis Encephalitis Viruses

Keywords

  • Alphavirus
  • Antibodies
  • Arbovirus
  • Bats
  • Flavivirus
  • Seroepidemiology
  • Trinidad

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Epidemiology
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Thompson, N. N., Auguste, A. J., Travassos da Rosa, A. P. A., Carrington, C. V. F., Blitvich, B. J., Chadee, D. D., ... Adesiyun, A. A. (2015). Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad. Zoonoses and Public Health, 62(1), 53-60. https://doi.org/10.1111/zph.12118

Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad. / Thompson, N. N.; Auguste, A. J.; Travassos da Rosa, A. P A; Carrington, C. V F; Blitvich, B. J.; Chadee, D. D.; Tesh, R. B.; Weaver, Scott; Adesiyun, A. A.

In: Zoonoses and Public Health, Vol. 62, No. 1, 01.02.2015, p. 53-60.

Research output: Contribution to journalArticle

Thompson, NN, Auguste, AJ, Travassos da Rosa, APA, Carrington, CVF, Blitvich, BJ, Chadee, DD, Tesh, RB, Weaver, S & Adesiyun, AA 2015, 'Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad', Zoonoses and Public Health, vol. 62, no. 1, pp. 53-60. https://doi.org/10.1111/zph.12118
Thompson NN, Auguste AJ, Travassos da Rosa APA, Carrington CVF, Blitvich BJ, Chadee DD et al. Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad. Zoonoses and Public Health. 2015 Feb 1;62(1):53-60. https://doi.org/10.1111/zph.12118
Thompson, N. N. ; Auguste, A. J. ; Travassos da Rosa, A. P A ; Carrington, C. V F ; Blitvich, B. J. ; Chadee, D. D. ; Tesh, R. B. ; Weaver, Scott ; Adesiyun, A. A. / Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad. In: Zoonoses and Public Health. 2015 ; Vol. 62, No. 1. pp. 53-60.
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abstract = "Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9{\%}) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3{\%}) and 3 (1.0{\%}) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6{\%}) bats. Seropositivity for TABV was significantly (P < 0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.",
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