Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad

N. N. Thompson; A. J. Auguste; A. P.A. Travassos da Rosa; C. V.F. Carrington; B. J. Blitvich; D. D. Chadee; R. B. Tesh; S. C. Weaver; A. A. Adesiyun

doi:10.1111/zph.12118

Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad

N. N. Thompson, A. J. Auguste, A. P.A. Travassos da Rosa, C. V.F. Carrington, B. J. Blitvich, D. D. Chadee, R. B. Tesh, S. C. Weaver, A. A. Adesiyun

Microbiology And Immunology

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26 Scopus citations

Abstract

Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P < 0.05; χ²) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ²) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.

Original language	English (US)
Pages (from-to)	53-60
Number of pages	8
Journal	Zoonoses and Public Health
Volume	62
Issue number	1
DOIs	https://doi.org/10.1111/zph.12118
State	Published - Feb 1 2015

Keywords

Alphavirus
Antibodies
Arbovirus
Bats
Flavivirus
Seroepidemiology
Trinidad

ASJC Scopus subject areas

Epidemiology
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1111/zph.12118

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title = "Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad",

abstract = "Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P < 0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.",

keywords = "Alphavirus, Antibodies, Arbovirus, Bats, Flavivirus, Seroepidemiology, Trinidad",

author = "Thompson, {N. N.} and Auguste, {A. J.} and {Travassos da Rosa}, {A. P.A.} and Carrington, {C. V.F.} and Blitvich, {B. J.} and Chadee, {D. D.} and Tesh, {R. B.} and Weaver, {S. C.} and Adesiyun, {A. A.}",

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day = "1",

doi = "10.1111/zph.12118",

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T1 - Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad

AU - Thompson, N. N.

AU - Auguste, A. J.

AU - Travassos da Rosa, A. P.A.

AU - Carrington, C. V.F.

AU - Blitvich, B. J.

AU - Chadee, D. D.

AU - Tesh, R. B.

AU - Weaver, S. C.

AU - Adesiyun, A. A.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P < 0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.

AB - Summary: A serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the Caribbean island of Trinidad. Sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (ELISAs) specific for antibodies against West Nile virus (WNV), Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), all of which are zoonotic viruses of public health significance in the region. Overall, the ELISAs resulted in the detection of VEEV-specific antibodies in 11 (2.9%) of 384 bats. Antibodies to WNV and EEEV were not detected in any sera. Of the 384 sera, 308 were also screened using hemagglutination inhibition assay (HIA) for antibodies to the aforementioned viruses as well as St. Louis encephalitis virus (SLEV; which also causes epidemic disease in humans), Rio Bravo virus (RBV), Tamana bat virus (TABV) and western equine encephalitis virus (WEEV). Using this approach, antibodies to TABV and RBV were detected in 47 (15.3%) and 3 (1.0%) bats, respectively. HIA results also suggest the presence of antibodies to an undetermined flavivirus(es) in 8 (2.6%) bats. Seropositivity for TABV was significantly (P < 0.05; χ2) associated with bat species, location and feeding preference, and for VEEV with roost type and location. Differences in prevalence rates between urban and rural locations were statistically significant (P < 0.05; χ2) for TABV only. None of the aforementioned factors was significantly associated with RBV seropositivity rates.

KW - Alphavirus

KW - Antibodies

KW - Arbovirus

KW - Bats

KW - Flavivirus

KW - Seroepidemiology

KW - Trinidad

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DO - 10.1111/zph.12118

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SN - 1863-1959

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JO - Zoonoses and Public Health

JF - Zoonoses and Public Health

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Seroepidemiology of selected alphaviruses and flaviviruses in bats in Trinidad

Abstract

Keywords

ASJC Scopus subject areas

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