Serologic diagnosis of West Nile virus infection

Pei-Yong Shi, Susan J. Wong

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The epidemic of West Nile virus (WNV) in the USA in 2002 represents the largest outbreak of meningoencephalitis in the Western Hemisphere ever reported. Besides natural transmission by mosquitoes, five new modes of WNV transmission to humans have been reported: blood transfusion, organ transplantation, transplacental transmission, breastfeeding and laboratory-acquired infection. The recognition of these new transmission routes has made the development of rapid and accurate serological diagnosis of WNV infection a public health priority. In this article, the current serologic assays for WNV diagnosis are reviewed, including immunoglobulin M antibody-capture ELISA, immunoglobulin G ELISA, indirect fluorescent antibody tests, hemagglutination inhibition tests and plaque reduction neutralization tests. The recently developed immunoassays that use purified recombinant envelope and nonstructural protein 5 of WNV as antigens are also reviewed. The nonstructural protein 5 protein-based assay can reliably discriminate between WNV and dengue or St. Louis encephalitis virus, as well as between natural WNV infection and flavivirus vaccination.

Original languageEnglish (US)
Pages (from-to)733-741
Number of pages9
JournalExpert Review of Molecular Diagnostics
Volume3
Issue number6
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

West Nile virus
Virus Diseases
St. Louis Encephalitis Viruses
Enzyme-Linked Immunosorbent Assay
Laboratory Infection
Hemagglutination Inhibition Tests
Flavivirus
Health Priorities
Neutralization Tests
Proteins
Meningoencephalitis
Dengue
Antibodies
Organ Transplantation
Breast Feeding
Culicidae
Immunoassay
Blood Transfusion
Disease Outbreaks
Immunoglobulin M

Keywords

  • Arbovirus
  • Diagnosis
  • Flavivirus
  • Serologic diagnosis
  • West Nile virus

ASJC Scopus subject areas

  • Genetics

Cite this

Serologic diagnosis of West Nile virus infection. / Shi, Pei-Yong; Wong, Susan J.

In: Expert Review of Molecular Diagnostics, Vol. 3, No. 6, 11.2003, p. 733-741.

Research output: Contribution to journalArticle

@article{9b321a0eaeb54f5b9ec159fe5cc4edef,
title = "Serologic diagnosis of West Nile virus infection",
abstract = "The epidemic of West Nile virus (WNV) in the USA in 2002 represents the largest outbreak of meningoencephalitis in the Western Hemisphere ever reported. Besides natural transmission by mosquitoes, five new modes of WNV transmission to humans have been reported: blood transfusion, organ transplantation, transplacental transmission, breastfeeding and laboratory-acquired infection. The recognition of these new transmission routes has made the development of rapid and accurate serological diagnosis of WNV infection a public health priority. In this article, the current serologic assays for WNV diagnosis are reviewed, including immunoglobulin M antibody-capture ELISA, immunoglobulin G ELISA, indirect fluorescent antibody tests, hemagglutination inhibition tests and plaque reduction neutralization tests. The recently developed immunoassays that use purified recombinant envelope and nonstructural protein 5 of WNV as antigens are also reviewed. The nonstructural protein 5 protein-based assay can reliably discriminate between WNV and dengue or St. Louis encephalitis virus, as well as between natural WNV infection and flavivirus vaccination.",
keywords = "Arbovirus, Diagnosis, Flavivirus, Serologic diagnosis, West Nile virus",
author = "Pei-Yong Shi and Wong, {Susan J.}",
year = "2003",
month = "11",
doi = "10.1586/14737159.3.6.733",
language = "English (US)",
volume = "3",
pages = "733--741",
journal = "Expert Review of Molecular Diagnostics",
issn = "1473-7159",
publisher = "Expert Reviews Ltd.",
number = "6",

}

TY - JOUR

T1 - Serologic diagnosis of West Nile virus infection

AU - Shi, Pei-Yong

AU - Wong, Susan J.

PY - 2003/11

Y1 - 2003/11

N2 - The epidemic of West Nile virus (WNV) in the USA in 2002 represents the largest outbreak of meningoencephalitis in the Western Hemisphere ever reported. Besides natural transmission by mosquitoes, five new modes of WNV transmission to humans have been reported: blood transfusion, organ transplantation, transplacental transmission, breastfeeding and laboratory-acquired infection. The recognition of these new transmission routes has made the development of rapid and accurate serological diagnosis of WNV infection a public health priority. In this article, the current serologic assays for WNV diagnosis are reviewed, including immunoglobulin M antibody-capture ELISA, immunoglobulin G ELISA, indirect fluorescent antibody tests, hemagglutination inhibition tests and plaque reduction neutralization tests. The recently developed immunoassays that use purified recombinant envelope and nonstructural protein 5 of WNV as antigens are also reviewed. The nonstructural protein 5 protein-based assay can reliably discriminate between WNV and dengue or St. Louis encephalitis virus, as well as between natural WNV infection and flavivirus vaccination.

AB - The epidemic of West Nile virus (WNV) in the USA in 2002 represents the largest outbreak of meningoencephalitis in the Western Hemisphere ever reported. Besides natural transmission by mosquitoes, five new modes of WNV transmission to humans have been reported: blood transfusion, organ transplantation, transplacental transmission, breastfeeding and laboratory-acquired infection. The recognition of these new transmission routes has made the development of rapid and accurate serological diagnosis of WNV infection a public health priority. In this article, the current serologic assays for WNV diagnosis are reviewed, including immunoglobulin M antibody-capture ELISA, immunoglobulin G ELISA, indirect fluorescent antibody tests, hemagglutination inhibition tests and plaque reduction neutralization tests. The recently developed immunoassays that use purified recombinant envelope and nonstructural protein 5 of WNV as antigens are also reviewed. The nonstructural protein 5 protein-based assay can reliably discriminate between WNV and dengue or St. Louis encephalitis virus, as well as between natural WNV infection and flavivirus vaccination.

KW - Arbovirus

KW - Diagnosis

KW - Flavivirus

KW - Serologic diagnosis

KW - West Nile virus

UR - http://www.scopus.com/inward/record.url?scp=0242641480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242641480&partnerID=8YFLogxK

U2 - 10.1586/14737159.3.6.733

DO - 10.1586/14737159.3.6.733

M3 - Article

C2 - 14628901

AN - SCOPUS:0242641480

VL - 3

SP - 733

EP - 741

JO - Expert Review of Molecular Diagnostics

JF - Expert Review of Molecular Diagnostics

SN - 1473-7159

IS - 6

ER -