Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis

Translated title of the contribution: Serological diagnosis of canine monocytic ehrlichiosis with Brazilian antigen of Ehrlichia canis

Daniel Moura Aguiar, Tais Saito, Mitika Kuribayashi Hagiwara, Rosângela Zacarias Machado, Marcelo Bahia Labruna

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The present study describes a successful isolation of Ehrlichia canis and its establishment in DH82 cells, followed by the development of an Indirect Fluorescent Antibodies Test (IFAT). Leukocytes collected from an experimentally infected dog with the Jaboticabal strain of E. canis were used to inoculate a DH82 cell monolayer. Two weeks later, the inoculated culture was checked for infectivity, every 5-6 days by both cytological staining and PCR, targeting a fragment of the dsb gene. The cell culture showed to be infected by Ehrlichia on day 27 by PCR and on day 28 by cytological staining. By the day 33, the infection rate reached 20% and on day 53, 60%. Currently, the isolate is established in DH82 cells, with several passages reaching 90-100% of infected cells, within 7 to 10 days post inoculation. After sequencing, the amplicon was identical to other E. canis corresponding sequences available in the GenBank. DH82 infected cells were used to standardize an IFAT for the diagnosis of canine ehrlichiosis.

Original languageUndefined
Pages (from-to)796-802
Number of pages7
JournalCiencia Rural
Volume37
Issue number3
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Ehrlichia canis
Ehrlichiosis
ehrlichiosis
serodiagnosis
Canidae
antigens
Antigens
dogs
cells
Ehrlichia
Staining and Labeling
Polymerase Chain Reaction
antibodies
Antibodies
Nucleic Acid Databases
leukocytes
cell culture
Leukocytes
pathogenicity
Cell Culture Techniques

Keywords

  • DH82
  • Ehrlichia canis
  • Immunofluorescence
  • Isolation
  • Serology

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Animal Science and Zoology
  • veterinary(all)

Cite this

Aguiar, D. M., Saito, T., Hagiwara, M. K., Machado, R. Z., & Labruna, M. B. (2007). Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. Ciencia Rural, 37(3), 796-802.

Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. / Aguiar, Daniel Moura; Saito, Tais; Hagiwara, Mitika Kuribayashi; Machado, Rosângela Zacarias; Labruna, Marcelo Bahia.

In: Ciencia Rural, Vol. 37, No. 3, 06.2007, p. 796-802.

Research output: Contribution to journalArticle

Aguiar, DM, Saito, T, Hagiwara, MK, Machado, RZ & Labruna, MB 2007, 'Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis', Ciencia Rural, vol. 37, no. 3, pp. 796-802.
Aguiar DM, Saito T, Hagiwara MK, Machado RZ, Labruna MB. Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. Ciencia Rural. 2007 Jun;37(3):796-802.
Aguiar, Daniel Moura ; Saito, Tais ; Hagiwara, Mitika Kuribayashi ; Machado, Rosângela Zacarias ; Labruna, Marcelo Bahia. / Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. In: Ciencia Rural. 2007 ; Vol. 37, No. 3. pp. 796-802.
@article{3a6b20dfb23a43ac942f417b38f30c56,
title = "Diagn{\'o}stico sorol{\'o}gico de erliquiose canina com ant{\'i}geno brasileiro de Ehrlichia canis",
abstract = "The present study describes a successful isolation of Ehrlichia canis and its establishment in DH82 cells, followed by the development of an Indirect Fluorescent Antibodies Test (IFAT). Leukocytes collected from an experimentally infected dog with the Jaboticabal strain of E. canis were used to inoculate a DH82 cell monolayer. Two weeks later, the inoculated culture was checked for infectivity, every 5-6 days by both cytological staining and PCR, targeting a fragment of the dsb gene. The cell culture showed to be infected by Ehrlichia on day 27 by PCR and on day 28 by cytological staining. By the day 33, the infection rate reached 20{\%} and on day 53, 60{\%}. Currently, the isolate is established in DH82 cells, with several passages reaching 90-100{\%} of infected cells, within 7 to 10 days post inoculation. After sequencing, the amplicon was identical to other E. canis corresponding sequences available in the GenBank. DH82 infected cells were used to standardize an IFAT for the diagnosis of canine ehrlichiosis.",
keywords = "DH82, Ehrlichia canis, Immunofluorescence, Isolation, Serology",
author = "Aguiar, {Daniel Moura} and Tais Saito and Hagiwara, {Mitika Kuribayashi} and Machado, {Ros{\^a}ngela Zacarias} and Labruna, {Marcelo Bahia}",
year = "2007",
month = "6",
language = "Undefined",
volume = "37",
pages = "796--802",
journal = "Ciencia Rural",
issn = "0103-8478",
publisher = "Universidade Federal de Santa Maria",
number = "3",

}

TY - JOUR

T1 - Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis

AU - Aguiar, Daniel Moura

AU - Saito, Tais

AU - Hagiwara, Mitika Kuribayashi

AU - Machado, Rosângela Zacarias

AU - Labruna, Marcelo Bahia

PY - 2007/6

Y1 - 2007/6

N2 - The present study describes a successful isolation of Ehrlichia canis and its establishment in DH82 cells, followed by the development of an Indirect Fluorescent Antibodies Test (IFAT). Leukocytes collected from an experimentally infected dog with the Jaboticabal strain of E. canis were used to inoculate a DH82 cell monolayer. Two weeks later, the inoculated culture was checked for infectivity, every 5-6 days by both cytological staining and PCR, targeting a fragment of the dsb gene. The cell culture showed to be infected by Ehrlichia on day 27 by PCR and on day 28 by cytological staining. By the day 33, the infection rate reached 20% and on day 53, 60%. Currently, the isolate is established in DH82 cells, with several passages reaching 90-100% of infected cells, within 7 to 10 days post inoculation. After sequencing, the amplicon was identical to other E. canis corresponding sequences available in the GenBank. DH82 infected cells were used to standardize an IFAT for the diagnosis of canine ehrlichiosis.

AB - The present study describes a successful isolation of Ehrlichia canis and its establishment in DH82 cells, followed by the development of an Indirect Fluorescent Antibodies Test (IFAT). Leukocytes collected from an experimentally infected dog with the Jaboticabal strain of E. canis were used to inoculate a DH82 cell monolayer. Two weeks later, the inoculated culture was checked for infectivity, every 5-6 days by both cytological staining and PCR, targeting a fragment of the dsb gene. The cell culture showed to be infected by Ehrlichia on day 27 by PCR and on day 28 by cytological staining. By the day 33, the infection rate reached 20% and on day 53, 60%. Currently, the isolate is established in DH82 cells, with several passages reaching 90-100% of infected cells, within 7 to 10 days post inoculation. After sequencing, the amplicon was identical to other E. canis corresponding sequences available in the GenBank. DH82 infected cells were used to standardize an IFAT for the diagnosis of canine ehrlichiosis.

KW - DH82

KW - Ehrlichia canis

KW - Immunofluorescence

KW - Isolation

KW - Serology

UR - http://www.scopus.com/inward/record.url?scp=34250163928&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250163928&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:34250163928

VL - 37

SP - 796

EP - 802

JO - Ciencia Rural

JF - Ciencia Rural

SN - 0103-8478

IS - 3

ER -