Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis

Translated title of the contribution: Serological diagnosis of canine monocytic ehrlichiosis with Brazilian antigen of Ehrlichia canis

Daniel Moura Aguiar, Taís Berelli Saito, Mitika Kuribayashi Hagiwara, Rosângela Zacarias Machado, Marcelo Bahia Labruna

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The present study describes a successful isolation of Ehrlichia canis and its establishment in DH82 cells, followed by the development of an Indirect Fluorescent Antibodies Test (IFAT). Leukocytes collected from an experimentally infected dog with the Jaboticabal strain of E. canis were used to inoculate a DH82 cell monolayer. Two weeks later, the inoculated culture was checked for infectivity, every 5-6 days by both cytological staining and PCR, targeting a fragment of the dsb gene. The cell culture showed to be infected by Ehrlichia on day 27 by PCR and on day 28 by cytological staining. By the day 33, the infection rate reached 20% and on day 53, 60%. Currently, the isolate is established in DH82 cells, with several passages reaching 90-100% of infected cells, within 7 to 10 days post inoculation. After sequencing, the amplicon was identical to other E. canis corresponding sequences available in the GenBank. DH82 infected cells were used to standardize an IFAT for the diagnosis of canine ehrlichiosis.

Original languagePortuguese
Pages (from-to)796-802
Number of pages7
JournalCiencia Rural
Volume37
Issue number3
StatePublished - Jun 1 2007
Externally publishedYes

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Keywords

  • DH82
  • Ehrlichia canis
  • Immunofluorescence
  • Isolation
  • Serology

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Agronomy and Crop Science
  • veterinary(all)

Cite this

Aguiar, D. M., Saito, T. B., Hagiwara, M. K., Machado, R. Z., & Labruna, M. B. (2007). Diagnóstico sorológico de erliquiose canina com antígeno brasileiro de Ehrlichia canis. Ciencia Rural, 37(3), 796-802.