Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta

R. M. Scott, F. M. Feinsod, I. H. Allam, T. G. Ksiazek, C. J. Peters, B. A. Botros, M. A. Darwish

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

To determine the accuracy of serological methods in detecting Rift Valley fever (RVF) viral antibodies, we examined serum samples obtained from 418 sheep in the Nile Delta by using five tests. The plaque reduction neutralization test (PRNT) was considered the standard serological method against which the four other tests were compared. Twenty-four serum samples had RVF viral antibodies detected by PRNT. Hemagglutination inhibition and enzyme-linked immunosorbent assay antibodies to RVF virus were also present in the same 24 serum samples. Indirect immunofluorescence was less sensitive in comparison with PRNT, and complement fixation was the least sensitive. These results extend observations made with laboratory animals to a large field-collected group of Egyptian sheep.

Original languageEnglish (US)
Pages (from-to)612-614
Number of pages3
JournalJournal of Clinical Microbiology
Volume24
Issue number4
StatePublished - Nov 19 1986
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta'. Together they form a unique fingerprint.

  • Cite this

    Scott, R. M., Feinsod, F. M., Allam, I. H., Ksiazek, T. G., Peters, C. J., Botros, B. A., & Darwish, M. A. (1986). Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta. Journal of Clinical Microbiology, 24(4), 612-614.