Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta

R. M. Scott, F. M. Feinsod, I. H. Allam, Thomas Ksiazek, C. J. Peters, B. A. Botros, M. A. Darwish

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To determine the accuracy of serological methods in detecting Rift Valley fever (RVF) viral antibodies, we examined serum samples obtained from 418 sheep in the Nile Delta by using five tests. The plaque reduction neutralization test (PRNT) was considered the standard serological method against which the four other tests were compared. Twenty-four serum samples had RVF viral antibodies detected by PRNT. Hemagglutination inhibition and enzyme-linked immunosorbent assay antibodies to RVF virus were also present in the same 24 serum samples. Indirect immunofluorescence was less sensitive in comparison with PRNT, and complement fixation was the least sensitive. These results extend observations made with laboratory animals to a large field-collected group of Egyptian sheep.

Original languageEnglish (US)
Pages (from-to)612-614
Number of pages3
JournalJournal of Clinical Microbiology
Volume24
Issue number4
StatePublished - 1986
Externally publishedYes

Fingerprint

Rift Valley Fever
Viral Antibodies
Neutralization Tests
Serologic Tests
Sheep
Rift Valley fever virus
Serum
Laboratory Animals
Hemagglutination
Indirect Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Antibodies

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Scott, R. M., Feinsod, F. M., Allam, I. H., Ksiazek, T., Peters, C. J., Botros, B. A., & Darwish, M. A. (1986). Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta. Journal of Clinical Microbiology, 24(4), 612-614.

Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta. / Scott, R. M.; Feinsod, F. M.; Allam, I. H.; Ksiazek, Thomas; Peters, C. J.; Botros, B. A.; Darwish, M. A.

In: Journal of Clinical Microbiology, Vol. 24, No. 4, 1986, p. 612-614.

Research output: Contribution to journalArticle

Scott, RM, Feinsod, FM, Allam, IH, Ksiazek, T, Peters, CJ, Botros, BA & Darwish, MA 1986, 'Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta', Journal of Clinical Microbiology, vol. 24, no. 4, pp. 612-614.
Scott, R. M. ; Feinsod, F. M. ; Allam, I. H. ; Ksiazek, Thomas ; Peters, C. J. ; Botros, B. A. ; Darwish, M. A. / Serological tests for detecting Rift Valley fever viral antibodies in sheep from the Nile Delta. In: Journal of Clinical Microbiology. 1986 ; Vol. 24, No. 4. pp. 612-614.
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