Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15

Geoffrey A. Preidis, Heuy Ching Wang, Dorothy E. Lewis, Alejandro Castellanos-Gonzalez, Kathleen A. Rogers, Edward A. Graviss, Honorine D. Ward, A. Clinton White

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Cryptosporidiosis is an important cause of diarrhea worldwide. In normal hosts, infection is self-limited and associated with seroconversion and partial immunity to reinfection. Immunity is associated with interferon gamma (IFN-γ) production. Cryptosporidium surface proteins gp15 and gp40 are among the immunodominant proteins in terms of antibody responses. We asked the question of whether these antigens also stimulate production of IFN-γ in patients who have serologic evidence of prior infection. Whole blood from seropositive donors was stimulated with recombinant gp15 and gp 40 from Cryptosporidium hominis and Cryptosporidium parvum or His-tag controls. C hominis gp15 stimulated increased production of IFN-γ. By contrast, there was no significant increase after stimulation with C parvum gp15 or either gp40 preparation. IFN-γ production in response to C hominis gp15 was noted in both CD4+ and CD8+ cells. This highlights the potential for C hominis gp15 as a vaccine candidate for human cryptosporidiosis.

Original languageEnglish (US)
Pages (from-to)583-585
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number3
DOIs
StatePublished - Sep 2007

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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