Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15

Geoffrey A. Preidis, Heuy Ching Wang, Dorothy E. Lewis, Alejandro Castellanos, Kathleen A. Rogers, Edward A. Graviss, Honorine D. Ward, A. Clinton White

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cryptosporidiosis is an important cause of diarrhea worldwide. In normal hosts, infection is self-limited and associated with seroconversion and partial immunity to reinfection. Immunity is associated with interferon gamma (IFN-γ) production. Cryptosporidium surface proteins gp15 and gp40 are among the immunodominant proteins in terms of antibody responses. We asked the question of whether these antigens also stimulate production of IFN-γ in patients who have serologic evidence of prior infection. Whole blood from seropositive donors was stimulated with recombinant gp15 and gp 40 from Cryptosporidium hominis and Cryptosporidium parvum or His-tag controls. C hominis gp15 stimulated increased production of IFN-γ. By contrast, there was no significant increase after stimulation with C parvum gp15 or either gp40 preparation. IFN-γ production in response to C hominis gp15 was noted in both CD4+ and CD8+ cells. This highlights the potential for C hominis gp15 as a vaccine candidate for human cryptosporidiosis.

Original languageEnglish (US)
Pages (from-to)583-585
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number3
StatePublished - Sep 2007

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Cryptosporidium
Interferon-gamma
Cryptosporidiosis
Immunity
Cryptosporidium parvum
Infection
Blood Donors
Antibody Formation
Diarrhea
Membrane Proteins
Vaccines
Antigens
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15. / Preidis, Geoffrey A.; Wang, Heuy Ching; Lewis, Dorothy E.; Castellanos, Alejandro; Rogers, Kathleen A.; Graviss, Edward A.; Ward, Honorine D.; White, A. Clinton.

In: American Journal of Tropical Medicine and Hygiene, Vol. 77, No. 3, 09.2007, p. 583-585.

Research output: Contribution to journalArticle

Preidis, Geoffrey A. ; Wang, Heuy Ching ; Lewis, Dorothy E. ; Castellanos, Alejandro ; Rogers, Kathleen A. ; Graviss, Edward A. ; Ward, Honorine D. ; White, A. Clinton. / Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15. In: American Journal of Tropical Medicine and Hygiene. 2007 ; Vol. 77, No. 3. pp. 583-585.
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