TY - JOUR
T1 - Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas
AU - Adetunji, Shakirat A.
AU - Krecek, Rosina C.
AU - Castellanos, Gabrielle
AU - Morrill, John C.
AU - Blue-McLendon, Alice
AU - Cook, Walt E.
AU - Esteve-Gassent, Maria D.
N1 - Funding Information:
We thank the following for their financial support: Texas A&M AgriLife Research : “Molecular ecology of vector-borne zoonosis in the Gulf of Mexico: A One Health approach”, “Improving diagnostic methods for Lyme Disease, and epidemiology of human and animal infections in TX”, project TEXV6579 (I-9524); Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University for graduate degree stipends; the TAMU Lyme Lab team; and Orion Research and Management Inc. We also thank Dr. Michael Criscitiello for critical reading of the manuscript.
Publisher Copyright:
© 2016 The Authors.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting seasons, using indirect ELISA. Samples with higher sero-reactivity (0.803 and above) than the negative control group (0.662) were further tested using a more specific standardized western immunoblot assay to rule out false positives. Using ELISA, 4.7% of the samples were sero-reactive against B. burgdorferi, and these originated in two eco-regions in Texas (Edwards Plateau and South Texas Plains). However, only 0.5% of the total samples were sero-reactive by standardized western immunoblot assay. Additionally, both ELISA and standardized western immunoblot assay results correlated with an increased incidence in human Lyme Disease cases reported in Texas. This is the first longitudinal study to demonstrate fluctuation in sero-reactivity of white-tailed deer to B. burgdorferi sensu stricto antigens in southern United States. Future ecological and geographical studies are needed to assess the environmental factors governing the prevalence of Lyme Disease in non-endemic areas of the southern United States.
AB - Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting seasons, using indirect ELISA. Samples with higher sero-reactivity (0.803 and above) than the negative control group (0.662) were further tested using a more specific standardized western immunoblot assay to rule out false positives. Using ELISA, 4.7% of the samples were sero-reactive against B. burgdorferi, and these originated in two eco-regions in Texas (Edwards Plateau and South Texas Plains). However, only 0.5% of the total samples were sero-reactive by standardized western immunoblot assay. Additionally, both ELISA and standardized western immunoblot assay results correlated with an increased incidence in human Lyme Disease cases reported in Texas. This is the first longitudinal study to demonstrate fluctuation in sero-reactivity of white-tailed deer to B. burgdorferi sensu stricto antigens in southern United States. Future ecological and geographical studies are needed to assess the environmental factors governing the prevalence of Lyme Disease in non-endemic areas of the southern United States.
KW - Borrelia burgdorferi
KW - Ixodes scapularis
KW - Lyme disease
KW - Sero-reactivity
KW - Texas
KW - White-tailed deer
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U2 - 10.1016/j.ijppaw.2016.06.002
DO - 10.1016/j.ijppaw.2016.06.002
M3 - Article
C2 - 27366674
AN - SCOPUS:84975479758
SN - 2213-2244
VL - 5
SP - 168
EP - 174
JO - International Journal for Parasitology: Parasites and Wildlife
JF - International Journal for Parasitology: Parasites and Wildlife
IS - 2
ER -