Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas

Shakirat A. Adetunji, Rosina C. Krecek, Gabrielle Castellanos, John C. Morrill, Alice Blue-McLendon, Walt E. Cook, Maria D. Esteve-Gassent

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting seasons, using indirect ELISA. Samples with higher sero-reactivity (0.803 and above) than the negative control group (0.662) were further tested using a more specific standardized western immunoblot assay to rule out false positives. Using ELISA, 4.7% of the samples were sero-reactive against B. burgdorferi, and these originated in two eco-regions in Texas (Edwards Plateau and South Texas Plains). However, only 0.5% of the total samples were sero-reactive by standardized western immunoblot assay. Additionally, both ELISA and standardized western immunoblot assay results correlated with an increased incidence in human Lyme Disease cases reported in Texas. This is the first longitudinal study to demonstrate fluctuation in sero-reactivity of white-tailed deer to B. burgdorferi sensu stricto antigens in southern United States. Future ecological and geographical studies are needed to assess the environmental factors governing the prevalence of Lyme Disease in non-endemic areas of the southern United States.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalInternational Journal for Parasitology: Parasites and Wildlife
Volume5
Issue number2
DOIs
StatePublished - Aug 1 2016

Fingerprint

Borrelia burgdorferi
Deer
Seroepidemiologic Studies
Odocoileus virginianus
seroprevalence
Lyme disease
Lyme Disease
antibodies
Antibodies
Western Blotting
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Southeastern United States
assays
arthropod-borne diseases
sampling
Ixodes
Ixodes scapularis
Arthropods
Ticks

Keywords

  • Borrelia burgdorferi
  • Ixodes scapularis
  • Lyme disease
  • Sero-reactivity
  • Texas
  • White-tailed deer

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology
  • Animal Science and Zoology

Cite this

Adetunji, S. A., Krecek, R. C., Castellanos, G., Morrill, J. C., Blue-McLendon, A., Cook, W. E., & Esteve-Gassent, M. D. (2016). Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas. International Journal for Parasitology: Parasites and Wildlife, 5(2), 168-174. https://doi.org/10.1016/j.ijppaw.2016.06.002

Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas. / Adetunji, Shakirat A.; Krecek, Rosina C.; Castellanos, Gabrielle; Morrill, John C.; Blue-McLendon, Alice; Cook, Walt E.; Esteve-Gassent, Maria D.

In: International Journal for Parasitology: Parasites and Wildlife, Vol. 5, No. 2, 01.08.2016, p. 168-174.

Research output: Contribution to journalArticle

Adetunji, SA, Krecek, RC, Castellanos, G, Morrill, JC, Blue-McLendon, A, Cook, WE & Esteve-Gassent, MD 2016, 'Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas', International Journal for Parasitology: Parasites and Wildlife, vol. 5, no. 2, pp. 168-174. https://doi.org/10.1016/j.ijppaw.2016.06.002
Adetunji, Shakirat A. ; Krecek, Rosina C. ; Castellanos, Gabrielle ; Morrill, John C. ; Blue-McLendon, Alice ; Cook, Walt E. ; Esteve-Gassent, Maria D. / Seroprevalence of Borrelia burgdorferi antibodies in white-tailed deer from Texas. In: International Journal for Parasitology: Parasites and Wildlife. 2016 ; Vol. 5, No. 2. pp. 168-174.
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abstract = "Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease in the United States. To determine the seroprevalence of B. burgdorferi antibodies in white-tailed deer (Odocoileus virginianus) from Texas, we analyzed serum samples (n = 1493) collected during the 2001-2015 hunting seasons, using indirect ELISA. Samples with higher sero-reactivity (0.803 and above) than the negative control group (0.662) were further tested using a more specific standardized western immunoblot assay to rule out false positives. Using ELISA, 4.7{\%} of the samples were sero-reactive against B. burgdorferi, and these originated in two eco-regions in Texas (Edwards Plateau and South Texas Plains). However, only 0.5{\%} of the total samples were sero-reactive by standardized western immunoblot assay. Additionally, both ELISA and standardized western immunoblot assay results correlated with an increased incidence in human Lyme Disease cases reported in Texas. This is the first longitudinal study to demonstrate fluctuation in sero-reactivity of white-tailed deer to B. burgdorferi sensu stricto antigens in southern United States. Future ecological and geographical studies are needed to assess the environmental factors governing the prevalence of Lyme Disease in non-endemic areas of the southern United States.",
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