Serosal bicarbonate protects against acid injury to rabbit esophagus

N. A. Tobey, D. W. Powell, V. J. Schreiner, R. C. Orlando

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The role of serosal bicarbonate ions (HCO3 -) in protection against acid injury was investigated in rabbit esophageal mucosa mounted in Ussing chambers. Luminal acidification reduced potential difference and resistance in tissues exposed serosally to HCO3 - or (unbuffered) HCO3-free solution. Whereas resistance declined similarly in both groups, potential difference declined less in HCO3 - solution. After washout, HCO3-bathed tissues also had a greater increase in resistance, lower permeability to mannitol, and less histologic damage. Furthermore, as protection by HCO3 - was not blocked by pretreatment with either the anion exchange blocker, 4 acetamido-4′-isothiocyanatostilbene 2-2′-disulfonic acid, or the carbonic anhydrase inhibitor, acetazolamide, and replacement of HCO3 - with N-2-hydroxy-ethylpiperazine-N′-2-ethane sulfonic acid, a buffer impermeant to cells, was protective, an extracellular site for protection by HCO3 - was likely. Where in the extracellular space HCO3 - buffers H+ is unclear, but the absence of change in luminal pH and the inability to prevent the acid-induced increase in permeability in HCO3-bathed tissues argue against a luminal (preepithelial) site. Also, rapid repair was not demonstrated, indicating that a luminal site for protection after surface cell damage was unlikely. We conclude that serosal HCO3 - is important in esophageal protection against acid damage by buffering H+ within the intercellular compartment of the extracellular space.

Original languageEnglish (US)
Pages (from-to)1466-1477
Number of pages12
JournalGastroenterology
Volume96
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Bicarbonates
Esophagus
Rabbits
Acids
Wounds and Injuries
Extracellular Space
Permeability
Buffers
Carbonic Anhydrase Inhibitors
Acetazolamide
Ethane
Sulfonic Acids
Mannitol
Anions

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Tobey, N. A., Powell, D. W., Schreiner, V. J., & Orlando, R. C. (1989). Serosal bicarbonate protects against acid injury to rabbit esophagus. Gastroenterology, 96(6), 1466-1477.

Serosal bicarbonate protects against acid injury to rabbit esophagus. / Tobey, N. A.; Powell, D. W.; Schreiner, V. J.; Orlando, R. C.

In: Gastroenterology, Vol. 96, No. 6, 1989, p. 1466-1477.

Research output: Contribution to journalArticle

Tobey, NA, Powell, DW, Schreiner, VJ & Orlando, RC 1989, 'Serosal bicarbonate protects against acid injury to rabbit esophagus', Gastroenterology, vol. 96, no. 6, pp. 1466-1477.
Tobey NA, Powell DW, Schreiner VJ, Orlando RC. Serosal bicarbonate protects against acid injury to rabbit esophagus. Gastroenterology. 1989;96(6):1466-1477.
Tobey, N. A. ; Powell, D. W. ; Schreiner, V. J. ; Orlando, R. C. / Serosal bicarbonate protects against acid injury to rabbit esophagus. In: Gastroenterology. 1989 ; Vol. 96, No. 6. pp. 1466-1477.
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