Serotonin receptor involvement in effects of restraint on female rat lordosis behavior

Lynda Uphouse, Cindy Hiegel, Erika Perez, Jutatip Guptarak

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Ovariectomized Fischer (CDF-344) rats, with bilateral cannulae in the mediobasal hypothalamus (MBH) near the ventromedial nucleus of the hypothalamus (VMN), were used to test the hypothesis that serotonin receptors in the VMN contribute to the lordosis-inhibiting effects of mild restraint. Rats were hormonally primed with 10 μg estradiol benzoate (EB) followed 48 h later with sesame seed oil. Four to six hours later (during the dark portion of the light-dark cycle), rats were pretested for sexual behavior. Thereafter, they were infused with saline, 2 μg of the serotonin (5-HT) 2 receptor agonist, (±)-2,5-dimethoxy-4-iodophenyl-2-aminopropane HCl (DOI), or 1 μg of the 5-HT1A receptor antagonist, N-{2[4-(2-methoxyphenyl)-1-piperazinyl]ethyl}-N-(2-pyridinyl) cyclohexanecarboxamide trihydrochloride (WAY100635). After a 5 min restraint, rats were tested for sexual receptivity. Rats infused with saline showed a significant decline in lordosis behavior after restraint. Infusion with either DOI or WAY100635 attenuated these effects of restraint. These findings extend earlier observations that the lordosis-disruptive effects of mild restraint include activation of 5-HT1A receptors in the VMN and are the first to implicate VMN 5-HT2 receptors in protection against mild restraint.

Original languageEnglish (US)
Pages (from-to)631-636
Number of pages6
JournalPharmacology Biochemistry and Behavior
Volume86
Issue number4
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

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Keywords

  • Female rats
  • Lordosis
  • Serotonin
  • Serotonin 1A receptors
  • Serotonin 2 receptors
  • Ventromedial nucleus of the hypothalamus

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

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