Serotyping Coxiella burnetii isolates from acute and chronic Q fever patients by using monoclonal antibodies

Yu Xuejie Yu, Didier Raoult

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Four mouse monoclonal antibodies reacting with Coxiella burnetii lipopolysaccharide antigens were produced and used in serotyping 17 C. burnetii isolates from acute Q fever and Q fever endocarditis patients in France. Two monoclonal antibodies (1B2 and 3B6) were considered specific for the Priscilla strain, a representative of Q fever endocarditis isolates, and did not react with the Nine Mile strain, which is representative of acute Q fever isolates. Monoclonal antibodies Nos. 1B2 and 3B6 reacted with 75% ( 3 4) acute Q fever isolates and 85% ( 11 13) of endocarditis isolates from France. It is reasonable to conclude that Priscilla-like strains cause both acute Q fever and Q fever endocarditis. The hypothesis that Priscilla-like strains only are associated with Q fever endocarditis should be reconsidered.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalFEMS Microbiology Letters
Volume117
Issue number1
StatePublished - Mar 15 1994
Externally publishedYes

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Keywords

  • Coxiella burnetii
  • Monoclonal antibodies
  • Q fever

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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