Serum TGF-β in thermally injured rats

Maryam Varedi, Marc G. Jeschke, Ella W. Englander, David N. Herndon, Robert E. Barrow

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A severe thermal injury is commonly associated with immune suppression and increased susceptibility to sepsis, frequently leading to multiple organ failure. Transforming growth factor-beta (TGF-β) is a potent immunosuppressive cytokine involved in complications associated with major trauma. Interleukin-4 (IL-4) is thought to synergize the immunosuppressive activity of TGF-β by promoting naive lymphocytes to differentiate and generate TGF-β secreting cells. This study examines the alterations in serum levels of TGF-β and IL-4 after a thermal injury. Male Sprague-Dawley rats (300-400 g) were anesthetized and received a 50% total body surface area full-thickness scald burn followed by fluid resuscitation and analgesia. Control rats were given the same treatment, but were immersed in water at room temperature. Rats were sacrificed from 1 h to 8 days after injury. Blood samples were collected aseptically from the inferior caval vein. Serum levels of TGF-β and IL-4 were measured by enzyme linked immunosorbent assay. Rats in the control and thermal injury groups showed similar increases in serum TGF-β 1 h after injury. A progressive increase in serum TGF-β was observed in burned animals compared to control animals starting on day 3 and continued through day 8 (P < 0.01). Serum IL-4 levels in control and thermally injured animals remained undetectable (<15.6 pg/mL) throughout the experiment. Thermal injury induces a significant increase in serum TGF-β, which may contribute to post-burn immunosuppression with an increased susceptibility to sepsis.

Original languageEnglish (US)
Pages (from-to)380-382
Number of pages3
JournalShock
Volume16
Issue number5
DOIs
StatePublished - Nov 2001

Keywords

  • Burned rat model
  • Immunosuppression
  • Serum cytokines
  • Thermal injury
  • Trauma

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

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    Varedi, M., Jeschke, M. G., Englander, E. W., Herndon, D. N., & Barrow, R. E. (2001). Serum TGF-β in thermally injured rats. Shock, 16(5), 380-382. https://doi.org/10.1097/00024382-200116050-00010