@article{132c352f693043b98f2ca8b41870954b,
title = "Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States",
abstract = "The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.",
keywords = "COVID-19, SARS-CoV-2, coronavirus",
author = "Jennifer Harcourt and Azaibi Tamin and Xiaoyan Lu and Shifaq Kamili and Sakthivel, {Senthil K.} and Janna Murray and Krista Queen and Ying Tao and Paden, {Clinton R.} and Jing Zhang and Yan Li and Anna Uehara and Haibin Wang and Cynthia Goldsmith and Bullock, {Hannah A.} and Lijuan Wang and Brett Whitaker and Brian Lynch and Rashi Gautam and Craig Schindewolf and Lokugamage, {Kumari G.} and Dionna Scharton and Jessica Plante and Divya Mirchandani and Widen, {Steven G.} and Krishna Narayanan and Shinji Makino and Ksiazek, {Thomas G.} and Plante, {Kenneth S.} and Weaver, {Scott C.} and Stephen Lindstrom and Suxiang Tong and Menachery, {Vineet D.} and Thornburg, {Natalie J.}",
note = "Funding Information: This study was supported by grants from the National Institute on Aging and the National Institutes of Allergy and Infectious Diseases of the National Institutes of Health (U19AI100625 and R00AG049092 to V.D.M., R24AI120942 to S.C.W., and AI99107 and AI114657 to S.M.); a STARs Award provided by the University of Texas System to V.D.M.; the Institute for Human Infections and Immunity at the University of Texas Medical Branch (S.M.); and trainee funding provided by the McLaughlin Fellowship Fund at the University of Texas Medical Branch. Publisher Copyright: {\textcopyright} 2020 Centers for Disease Control and Prevention (CDC). All rights reserved.",
year = "2020",
month = jun,
day = "1",
doi = "10.3201/EID2606.200516",
language = "English (US)",
volume = "26",
pages = "1266--1273",
journal = "Emerging infectious diseases",
issn = "1080-6040",
publisher = "Centers for Disease Control and Prevention (CDC)",
number = "6",
}