Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

Jennifer Harcourt, Azaibi Tamin, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig SchindewolfKumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery, Natalie J. Thornburg

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

Original languageEnglish (US)
Pages (from-to)1266-1273
Number of pages8
JournalEmerging infectious diseases
Volume26
Issue number6
DOIs
StatePublished - Jun 1 2020

Keywords

  • COVID-19
  • SARS-CoV-2
  • coronavirus

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

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  • Cite this

    Harcourt, J., Tamin, A., Lu, X., Kamili, S., Sakthivel, S. K., Murray, J., Queen, K., Tao, Y., Paden, C. R., Zhang, J., Li, Y., Uehara, A., Wang, H., Goldsmith, C., Bullock, H. A., Wang, L., Whitaker, B., Lynch, B., Gautam, R., ... Thornburg, N. J. (2020). Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States. Emerging infectious diseases, 26(6), 1266-1273. https://doi.org/10.3201/EID2606.200516