Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

Jennifer Harcourt; Azaibi Tamin; Xiaoyan Lu; Shifaq Kamili; Senthil K. Sakthivel; Janna Murray; Krista Queen; Ying Tao; Clinton R. Paden; Jing Zhang; Yan Li; Anna Uehara; Haibin Wang; Cynthia Goldsmith; Hannah A. Bullock; Lijuan Wang; Brett Whitaker; Brian Lynch; Rashi Gautam; Craig Schindewolf; Kumari G. Lokugamage; Dionna Scharton; Jessica A. Plante; Divya Mirchandani; Steven G. Widen; Krishna Narayanan; Shinji Makino; Thomas G. Ksiazek; Kenneth S. Plante; Scott C. Weaver; Stephen Lindstrom; Suxiang Tong; Vineet D. Menachery; Natalie J. Thornburg

doi:10.3201/EID2606.200516

Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

Jennifer Harcourt, Azaibi Tamin, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig SchindewolfKumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery, Natalie J. Thornburg

Research output: Contribution to journal › Article › peer-review

412 Scopus citations

Abstract

The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

Original language	English (US)
Pages (from-to)	1266-1273
Number of pages	8
Journal	Emerging infectious diseases
Volume	26
Issue number	6
DOIs	https://doi.org/10.3201/EID2606.200516
State	Published - Jun 1 2020

Keywords

COVID-19
SARS-CoV-2
coronavirus

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

Access to Document

10.3201/EID2606.200516

Cite this

Harcourt, J., Tamin, A., Lu, X., Kamili, S., Sakthivel, S. K., Murray, J., Queen, K., Tao, Y., Paden, C. R., Zhang, J., Li, Y., Uehara, A., Wang, H., Goldsmith, C., Bullock, H. A., Wang, L., Whitaker, B., Lynch, B., Gautam, R., ... Thornburg, N. J. (2020). Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States. Emerging infectious diseases, 26(6), 1266-1273. https://doi.org/10.3201/EID2606.200516

Harcourt, J, Tamin, A, Lu, X, Kamili, S, Sakthivel, SK, Murray, J, Queen, K, Tao, Y, Paden, CR, Zhang, J, Li, Y, Uehara, A, Wang, H, Goldsmith, C, Bullock, HA, Wang, L, Whitaker, B, Lynch, B, Gautam, R, Schindewolf, C, Lokugamage, KG, Scharton, D, Plante, JA, Mirchandani, D, Widen, SG, Narayanan, K, Makino, S , Ksiazek, TG , Plante, KS , Weaver, SC, Lindstrom, S, Tong, S, Menachery, VD & Thornburg, NJ 2020, 'Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States', Emerging infectious diseases, vol. 26, no. 6, pp. 1266-1273. https://doi.org/10.3201/EID2606.200516

@article{132c352f693043b98f2ca8b41870954b,

title = "Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States",

abstract = "The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.",

keywords = "COVID-19, SARS-CoV-2, coronavirus",

author = "Jennifer Harcourt and Azaibi Tamin and Xiaoyan Lu and Shifaq Kamili and Sakthivel, {Senthil K.} and Janna Murray and Krista Queen and Ying Tao and Paden, {Clinton R.} and Jing Zhang and Yan Li and Anna Uehara and Haibin Wang and Cynthia Goldsmith and Bullock, {Hannah A.} and Lijuan Wang and Brett Whitaker and Brian Lynch and Rashi Gautam and Craig Schindewolf and Lokugamage, {Kumari G.} and Dionna Scharton and Plante, {Jessica A.} and Divya Mirchandani and Widen, {Steven G.} and Krishna Narayanan and Shinji Makino and Ksiazek, {Thomas G.} and Plante, {Kenneth S.} and Weaver, {Scott C.} and Stephen Lindstrom and Suxiang Tong and Menachery, {Vineet D.} and Thornburg, {Natalie J.}",

year = "2020",

month = jun,

day = "1",

doi = "10.3201/EID2606.200516",

language = "English (US)",

volume = "26",

pages = "1266--1273",

journal = "Emerging infectious diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention (CDC)",

number = "6",

}

TY - JOUR

T1 - Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

AU - Harcourt, Jennifer

AU - Tamin, Azaibi

AU - Lu, Xiaoyan

AU - Kamili, Shifaq

AU - Sakthivel, Senthil K.

AU - Murray, Janna

AU - Queen, Krista

AU - Tao, Ying

AU - Paden, Clinton R.

AU - Zhang, Jing

AU - Li, Yan

AU - Uehara, Anna

AU - Wang, Haibin

AU - Goldsmith, Cynthia

AU - Bullock, Hannah A.

AU - Wang, Lijuan

AU - Whitaker, Brett

AU - Lynch, Brian

AU - Gautam, Rashi

AU - Schindewolf, Craig

AU - Lokugamage, Kumari G.

AU - Scharton, Dionna

AU - Plante, Jessica A.

AU - Mirchandani, Divya

AU - Widen, Steven G.

AU - Narayanan, Krishna

AU - Makino, Shinji

AU - Ksiazek, Thomas G.

AU - Plante, Kenneth S.

AU - Weaver, Scott C.

AU - Lindstrom, Stephen

AU - Tong, Suxiang

AU - Menachery, Vineet D.

AU - Thornburg, Natalie J.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

AB - The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

KW - COVID-19

KW - SARS-CoV-2

KW - coronavirus

UR - http://www.scopus.com/inward/record.url?scp=85082747770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85082747770&partnerID=8YFLogxK

U2 - 10.3201/EID2606.200516

DO - 10.3201/EID2606.200516

M3 - Article

C2 - 32160149

AN - SCOPUS:85082747770

SN - 1080-6040

VL - 26

SP - 1266

EP - 1273

JO - Emerging infectious diseases

JF - Emerging infectious diseases

IS - 6

ER -

Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this