Severe acute respiratory syndrome coronavirus 3a protein is released in membranous structures from 3a protein-expressing cells and infected cells

Cheng Huang, Krishna Narayanan, Naoto Ito, C. J. Peters, Shinji Makino

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Severe acute respiratory syndrome coronavirus (SCoV) accessory protein 3a is a virus structural protein. We demonstrate here that 3a protein was released efficiently in membranous structures from various cell lines expressing 3a protein. A subpopulation of the released 3a protein is associated with detergent-resistant membranes. The presence of the YxxΦ and diacidic motifs, located within the cytoplasmic tail of the 3a protein, was not required for its efficient release. Analysis of supernatant from SCoV-infected cells with sucrose gradient sedimentation and virus capture assay indicated that the 3a protein was released from infected cells in two distinct populations, as a component of SCoV particles, and in membrane structures with a lower buoyant density. These data provide new insights into the biological properties of SCoV 3a protein.

Original languageEnglish (US)
Pages (from-to)210-217
Number of pages8
JournalJournal of Virology
Volume80
Issue number1
DOIs
StatePublished - Jan 2006

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Coronavirus
Coronavirinae
Proteins
proteins
cells
Viral Structural Proteins
Membranes
viruses
structural proteins
Detergents
Sucrose
Severe acute respiratory syndrome coronavirus
3a protein, severe acute respiratory syndrome coronavirus
detergents
tail
Viruses
Cell Line
cell lines
sucrose
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Severe acute respiratory syndrome coronavirus 3a protein is released in membranous structures from 3a protein-expressing cells and infected cells. / Huang, Cheng; Narayanan, Krishna; Ito, Naoto; Peters, C. J.; Makino, Shinji.

In: Journal of Virology, Vol. 80, No. 1, 01.2006, p. 210-217.

Research output: Contribution to journalArticle

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