Severe acute respiratory syndrome coronavirus 7a accessory protein is a viral structural protein

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Abstract

Severe acute respiratory syndrome coronavirus (SCoV) 7a protein is one of the viral accessory proteins. In expressing cells, 7a protein exhibits a variety of biological activities, including induction of apoptosis, activation of the mitogen-activated protein kinase signaling pathway, inhibition of host protein translation, and suppression of cell growth progression. Analysis of SCoV particles that were purified by either sucrose gradient equilibrium centrifugation or a virus capture assay, in which intact SCoV particles were specifically immunoprecipitated by anti-S protein monoclonal antibody, demonstrated that 7a protein was associated with purified SCoV particles. Coexpression of 7a protein with SCoV S, M, N, and E proteins resulted in production of virus-like particles (VLPs) carrying 7a protein, while 7a protein was not released from cells expressing 7a protein alone. Although interaction between 7a protein and another SCoV accessory protein, 3a, has been reported, 3a protein was dispensable for assembly of 7a protein into VLPs. S protein was not required for the 7a protein incorporation into VLPs, and yet 7a protein interacted with S protein in coexpressing cells. These data established that, in addition to 3a protein, 7a protein was a SCoV accessory protein identified as a SCoV structural protein.

Original languageEnglish (US)
Pages (from-to)7287-7294
Number of pages8
JournalJournal of Virology
Volume80
Issue number15
DOIs
StatePublished - Aug 2006

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Viral Structural Proteins
viral proteins
structural proteins
Coronavirus
Proteins
proteins
Coronavirinae
Protein S
Virion
virus-like particles
Severe acute respiratory syndrome coronavirus
SARS virus sars7a protein
Viral Regulatory and Accessory Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Severe acute respiratory syndrome coronavirus 7a accessory protein is a viral structural protein. / Huang, Cheng; Ito, Naoto; Tseng, Chien-Te; Makino, Shinji.

In: Journal of Virology, Vol. 80, No. 15, 08.2006, p. 7287-7294.

Research output: Contribution to journalArticle

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