Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells

Cheng Huang, C. J. Peters, Shinji Makino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.

Original languageEnglish (US)
Pages (from-to)5423-5426
Number of pages4
JournalJournal of Virology
Volume81
Issue number10
DOIs
StatePublished - May 2007

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Severe Acute Respiratory Syndrome
Coronavirus
Coronavirinae
virion
Virion
Proteins
proteins
cells
virus-like particles
Severe acute respiratory syndrome coronavirus
Protein S
Centrifugation
centrifugation
Sucrose
plasmids
Plasmids
sucrose
Viruses
viruses
antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

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