Severe burn and disuse in the rat independently adversely impact body composition and adipokines

Charles E. Wade, Lisa A. Baer, Xiaowu Wu, David T. Silliman, Thomas J. Walters, Steven Wolf

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction: Severe trauma is accompanied by a period of hypermetabolism and disuse. In this study, a rat model was used to determine the effects of burn and disuse independently and in combination on body composition, food intake and adipokines.Methods: Male rats were assigned to four groups 1) sham ambulatory (SA), 2) sham hindlimb unloaded (SH), 3) 40% total body surface area full thickness scald burn ambulatory (BA) and 4) burn and hindlimb unloaded (BH). Animals designated to the SH and BH groups were placed in a tail traction system and their hindlimbs unloaded. Animals were followed for 14 days. Plasma, urine, fecal and tissue samples were analyzed.Results: SA had a progressive increase in body mass (BM), SH and BA no change and BH a reduction. Compared to SA, BM was reduced by 10% in both SH and BA and by 17% when combined in BH. Compared to SA, all groups had reductions in lean and fat body mass with BH being greater. The decrease in lean mass was associated with the rate of urinary corticosterone excretion. The loss in fat mass was associated with decreases in plasma leptin and adiponectin and an increase in ghrelin. Following the acute response to injury, BH had a greater food intake per 100 g BM. Food intake was associated with the levels of leptin, adiponectin and ghrelin.Conclusions: The effects of the combination of burn and disuse in this animal model were additive, therefore in assessing metabolic changes with severe trauma both injury and disuse should be considered. Furthermore, the observed changes in adipokines, corticosterone and ghrelin provide insights for interventions to attenuate the hypermetabolic state following injury, possibly reducing catabolism and muscle loss and subsequent adverse effects on recovery and function.

Original languageEnglish (US)
Article numberR225
JournalCritical Care
Volume17
Issue number5
DOIs
StatePublished - Oct 7 2013
Externally publishedYes

Fingerprint

Adipokines
Hindlimb
Body Composition
Ghrelin
Wounds and Injuries
Eating
Adiponectin
Corticosterone
Leptin
Fat Body
Body Surface Area
Recovery of Function
Traction
Tail
Animal Models
Fats
Urine
Muscles

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Severe burn and disuse in the rat independently adversely impact body composition and adipokines. / Wade, Charles E.; Baer, Lisa A.; Wu, Xiaowu; Silliman, David T.; Walters, Thomas J.; Wolf, Steven.

In: Critical Care, Vol. 17, No. 5, R225, 07.10.2013.

Research output: Contribution to journalArticle

Wade, Charles E. ; Baer, Lisa A. ; Wu, Xiaowu ; Silliman, David T. ; Walters, Thomas J. ; Wolf, Steven. / Severe burn and disuse in the rat independently adversely impact body composition and adipokines. In: Critical Care. 2013 ; Vol. 17, No. 5.
@article{bba79c3ff7594085a708e4c6f87f21bd,
title = "Severe burn and disuse in the rat independently adversely impact body composition and adipokines",
abstract = "Introduction: Severe trauma is accompanied by a period of hypermetabolism and disuse. In this study, a rat model was used to determine the effects of burn and disuse independently and in combination on body composition, food intake and adipokines.Methods: Male rats were assigned to four groups 1) sham ambulatory (SA), 2) sham hindlimb unloaded (SH), 3) 40{\%} total body surface area full thickness scald burn ambulatory (BA) and 4) burn and hindlimb unloaded (BH). Animals designated to the SH and BH groups were placed in a tail traction system and their hindlimbs unloaded. Animals were followed for 14 days. Plasma, urine, fecal and tissue samples were analyzed.Results: SA had a progressive increase in body mass (BM), SH and BA no change and BH a reduction. Compared to SA, BM was reduced by 10{\%} in both SH and BA and by 17{\%} when combined in BH. Compared to SA, all groups had reductions in lean and fat body mass with BH being greater. The decrease in lean mass was associated with the rate of urinary corticosterone excretion. The loss in fat mass was associated with decreases in plasma leptin and adiponectin and an increase in ghrelin. Following the acute response to injury, BH had a greater food intake per 100 g BM. Food intake was associated with the levels of leptin, adiponectin and ghrelin.Conclusions: The effects of the combination of burn and disuse in this animal model were additive, therefore in assessing metabolic changes with severe trauma both injury and disuse should be considered. Furthermore, the observed changes in adipokines, corticosterone and ghrelin provide insights for interventions to attenuate the hypermetabolic state following injury, possibly reducing catabolism and muscle loss and subsequent adverse effects on recovery and function.",
author = "Wade, {Charles E.} and Baer, {Lisa A.} and Xiaowu Wu and Silliman, {David T.} and Walters, {Thomas J.} and Steven Wolf",
year = "2013",
month = "10",
day = "7",
doi = "10.1186/cc13048",
language = "English (US)",
volume = "17",
journal = "Critical Care",
issn = "1364-8535",
publisher = "BioMed Central Ltd.",
number = "5",

}

TY - JOUR

T1 - Severe burn and disuse in the rat independently adversely impact body composition and adipokines

AU - Wade, Charles E.

AU - Baer, Lisa A.

AU - Wu, Xiaowu

AU - Silliman, David T.

AU - Walters, Thomas J.

AU - Wolf, Steven

PY - 2013/10/7

Y1 - 2013/10/7

N2 - Introduction: Severe trauma is accompanied by a period of hypermetabolism and disuse. In this study, a rat model was used to determine the effects of burn and disuse independently and in combination on body composition, food intake and adipokines.Methods: Male rats were assigned to four groups 1) sham ambulatory (SA), 2) sham hindlimb unloaded (SH), 3) 40% total body surface area full thickness scald burn ambulatory (BA) and 4) burn and hindlimb unloaded (BH). Animals designated to the SH and BH groups were placed in a tail traction system and their hindlimbs unloaded. Animals were followed for 14 days. Plasma, urine, fecal and tissue samples were analyzed.Results: SA had a progressive increase in body mass (BM), SH and BA no change and BH a reduction. Compared to SA, BM was reduced by 10% in both SH and BA and by 17% when combined in BH. Compared to SA, all groups had reductions in lean and fat body mass with BH being greater. The decrease in lean mass was associated with the rate of urinary corticosterone excretion. The loss in fat mass was associated with decreases in plasma leptin and adiponectin and an increase in ghrelin. Following the acute response to injury, BH had a greater food intake per 100 g BM. Food intake was associated with the levels of leptin, adiponectin and ghrelin.Conclusions: The effects of the combination of burn and disuse in this animal model were additive, therefore in assessing metabolic changes with severe trauma both injury and disuse should be considered. Furthermore, the observed changes in adipokines, corticosterone and ghrelin provide insights for interventions to attenuate the hypermetabolic state following injury, possibly reducing catabolism and muscle loss and subsequent adverse effects on recovery and function.

AB - Introduction: Severe trauma is accompanied by a period of hypermetabolism and disuse. In this study, a rat model was used to determine the effects of burn and disuse independently and in combination on body composition, food intake and adipokines.Methods: Male rats were assigned to four groups 1) sham ambulatory (SA), 2) sham hindlimb unloaded (SH), 3) 40% total body surface area full thickness scald burn ambulatory (BA) and 4) burn and hindlimb unloaded (BH). Animals designated to the SH and BH groups were placed in a tail traction system and their hindlimbs unloaded. Animals were followed for 14 days. Plasma, urine, fecal and tissue samples were analyzed.Results: SA had a progressive increase in body mass (BM), SH and BA no change and BH a reduction. Compared to SA, BM was reduced by 10% in both SH and BA and by 17% when combined in BH. Compared to SA, all groups had reductions in lean and fat body mass with BH being greater. The decrease in lean mass was associated with the rate of urinary corticosterone excretion. The loss in fat mass was associated with decreases in plasma leptin and adiponectin and an increase in ghrelin. Following the acute response to injury, BH had a greater food intake per 100 g BM. Food intake was associated with the levels of leptin, adiponectin and ghrelin.Conclusions: The effects of the combination of burn and disuse in this animal model were additive, therefore in assessing metabolic changes with severe trauma both injury and disuse should be considered. Furthermore, the observed changes in adipokines, corticosterone and ghrelin provide insights for interventions to attenuate the hypermetabolic state following injury, possibly reducing catabolism and muscle loss and subsequent adverse effects on recovery and function.

UR - http://www.scopus.com/inward/record.url?scp=84885008613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885008613&partnerID=8YFLogxK

U2 - 10.1186/cc13048

DO - 10.1186/cc13048

M3 - Article

C2 - 24099533

AN - SCOPUS:84885008613

VL - 17

JO - Critical Care

JF - Critical Care

SN - 1364-8535

IS - 5

M1 - R225

ER -