Severe burn-induced inflammation and remodeling of achilles tendon in a rat model

Paula Hernandez, Dustin Buller, Thomas Mitchell, Jamie Wright, Haixiang Liang, Kshitij Manchanda, Tre Welch, Ryan M. Huebinger, Deborah L. Carlson, Steven Wolf, Juquan Song

Research output: Contribution to journalArticle

Abstract

Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague-Dawley rats received 40% total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1β, TNF, MMP9, MMP13, TGFβ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1β as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFβ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats. We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalShock
Volume50
Issue number3
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Achilles Tendon
Burns
Tendons
Inflammation
Tendinopathy
Collagen
Interleukin-1
Interleukin-6
Bone and Bones
Muscles
Musculoskeletal System
Body Surface Area
Sprague Dawley Rats
Staining and Labeling
Gene Expression
Control Groups
Proteins

Keywords

  • Biomechanical property
  • Collagen alignment
  • Tendinopathy
  • Thermal injury

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Hernandez, P., Buller, D., Mitchell, T., Wright, J., Liang, H., Manchanda, K., ... Song, J. (2018). Severe burn-induced inflammation and remodeling of achilles tendon in a rat model. Shock, 50(3), 346-350. https://doi.org/10.1097/SHK.0000000000001037

Severe burn-induced inflammation and remodeling of achilles tendon in a rat model. / Hernandez, Paula; Buller, Dustin; Mitchell, Thomas; Wright, Jamie; Liang, Haixiang; Manchanda, Kshitij; Welch, Tre; Huebinger, Ryan M.; Carlson, Deborah L.; Wolf, Steven; Song, Juquan.

In: Shock, Vol. 50, No. 3, 01.01.2018, p. 346-350.

Research output: Contribution to journalArticle

Hernandez, P, Buller, D, Mitchell, T, Wright, J, Liang, H, Manchanda, K, Welch, T, Huebinger, RM, Carlson, DL, Wolf, S & Song, J 2018, 'Severe burn-induced inflammation and remodeling of achilles tendon in a rat model', Shock, vol. 50, no. 3, pp. 346-350. https://doi.org/10.1097/SHK.0000000000001037
Hernandez P, Buller D, Mitchell T, Wright J, Liang H, Manchanda K et al. Severe burn-induced inflammation and remodeling of achilles tendon in a rat model. Shock. 2018 Jan 1;50(3):346-350. https://doi.org/10.1097/SHK.0000000000001037
Hernandez, Paula ; Buller, Dustin ; Mitchell, Thomas ; Wright, Jamie ; Liang, Haixiang ; Manchanda, Kshitij ; Welch, Tre ; Huebinger, Ryan M. ; Carlson, Deborah L. ; Wolf, Steven ; Song, Juquan. / Severe burn-induced inflammation and remodeling of achilles tendon in a rat model. In: Shock. 2018 ; Vol. 50, No. 3. pp. 346-350.
@article{444d69217ab24be08563452a0a163215,
title = "Severe burn-induced inflammation and remodeling of achilles tendon in a rat model",
abstract = "Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague-Dawley rats received 40{\%} total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1β, TNF, MMP9, MMP13, TGFβ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1β as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFβ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats. We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.",
keywords = "Biomechanical property, Collagen alignment, Tendinopathy, Thermal injury",
author = "Paula Hernandez and Dustin Buller and Thomas Mitchell and Jamie Wright and Haixiang Liang and Kshitij Manchanda and Tre Welch and Huebinger, {Ryan M.} and Carlson, {Deborah L.} and Steven Wolf and Juquan Song",
year = "2018",
month = "1",
day = "1",
doi = "10.1097/SHK.0000000000001037",
language = "English (US)",
volume = "50",
pages = "346--350",
journal = "Shock",
issn = "1073-2322",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Severe burn-induced inflammation and remodeling of achilles tendon in a rat model

AU - Hernandez, Paula

AU - Buller, Dustin

AU - Mitchell, Thomas

AU - Wright, Jamie

AU - Liang, Haixiang

AU - Manchanda, Kshitij

AU - Welch, Tre

AU - Huebinger, Ryan M.

AU - Carlson, Deborah L.

AU - Wolf, Steven

AU - Song, Juquan

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague-Dawley rats received 40% total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1β, TNF, MMP9, MMP13, TGFβ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1β as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFβ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats. We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.

AB - Severe burn causes systemic inflammation and hypercatabolism, resulting in damage to multiple organs distant to the burn site, including the musculoskeletal system. Bone mass and muscle loss have been reported. However, tendon that connects bone and muscle has not been studied in comparable detail. Here we aimed to characterize the molecular and functional changes in Achilles tendon triggered by severe burn. Forty male Sprague-Dawley rats received 40% total body surface area scald burn. Achilles tendons were collected up to 14 days postburn. Sham-treated animals served as a control group. We analyzed tendons for changes in expression of IL-6, IL-1β, TNF, MMP9, MMP13, TGFβ1, Collagens I and III, and for morphological and biomechanical changes. Gene expression of IL-6 and IL-1β as well as MMP9 and MMP13 increased in rat tendon 3 days after burn. Col3a1 increased at day 3 and col1a1 at day 7. At day 14, TGFβ1 increased, whereas the protein ratio for collagens I/III decreased, indicating tendon remodeling. Histological analysis with H&E and Picrosirius red staining further revealed a decrease in organized collagen fibers 14 days after burn. Biomechanical analysis showed a decrease in stiffness and ultimate force of tendons in burn rats. We conclude that tendinopathy was observed in Achilles tendon 14 days after severe burn, via the induction of inflammation and remodeling. The present study provides a model of tendinopathy that may be used for the development of therapeutic approaches after burn.

KW - Biomechanical property

KW - Collagen alignment

KW - Tendinopathy

KW - Thermal injury

UR - http://www.scopus.com/inward/record.url?scp=85056521457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056521457&partnerID=8YFLogxK

U2 - 10.1097/SHK.0000000000001037

DO - 10.1097/SHK.0000000000001037

M3 - Article

VL - 50

SP - 346

EP - 350

JO - Shock

JF - Shock

SN - 1073-2322

IS - 3

ER -