Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue

Craig Porter, David Herndon, Nisha Bhattarai, John Ogunbileje, Bartosz Szczesny, Csaba Szabo, Tracy Toliver-Kinsky, Labros S. Sidossis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chronic cold exposure induces functionally thermogenic mitochondria in the inguinal white adipose tissue (iWAT) of mice. Whether this response occurs in pathophysiological states remains unclear. The purpose of this study was to determine the impact of severe burn trauma on iWAT mitochondrial function in mice. Male BALB/c mice (10-12 weeks) received full-thickness scald burns to ∼30% of the body surface area. Inguinal white adipose tissue was harvested from mice at 1, 4, 10, 20, and 40 days postinjury. Total and uncoupling protein 1 (UCP1)-dependent mitochondrial thermogenesis were determined in iWAT. Citrate synthase activity was determined as a proxy of mitochondrial abundance. Immunohistochemistry was performed to assess iWAT morphology and UCP1 expression. Uncoupling protein 1-dependent respiration was significantly greater at 4 and 10 days after burn compared with sham, peaking at 20 days after burn (P < 0.001). Citrate synthase activity was threefold greater at 4, 10, 20, and 40 days after burn versus sham (P < 0.05). Per mitochondrion, UCP1 function increased after burn trauma (P < 0.05). After burn trauma, iWAT exhibited numerous multilocular lipid droplets that stained positive for UCP1. The current findings demonstrate the induction of thermogenically competent mitochondria within rodent iWAT in a model of severe burn trauma. These data identify a specific pathology that induces the browning of white adipose tissue in vivo and may offer a mechanistic explanation for the chronic hypermetabolism observed in burn victims.

Original languageEnglish (US)
Pages (from-to)258-264
Number of pages7
JournalShock
Volume44
Issue number3
DOIs
StatePublished - Sep 18 2015

Fingerprint

White Adipose Tissue
Groin
Mitochondria
Wounds and Injuries
Citrate (si)-Synthase
Thermogenesis
Body Surface Area
Proxy
Burns
Rodentia
Respiration
Immunohistochemistry
Uncoupling Protein 1
Pathology

Keywords

  • Adipose tissue
  • burn injury
  • hypermetabolism
  • mitochondria
  • thermogenesis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue. / Porter, Craig; Herndon, David; Bhattarai, Nisha; Ogunbileje, John; Szczesny, Bartosz; Szabo, Csaba; Toliver-Kinsky, Tracy; Sidossis, Labros S.

In: Shock, Vol. 44, No. 3, 18.09.2015, p. 258-264.

Research output: Contribution to journalArticle

Porter, Craig ; Herndon, David ; Bhattarai, Nisha ; Ogunbileje, John ; Szczesny, Bartosz ; Szabo, Csaba ; Toliver-Kinsky, Tracy ; Sidossis, Labros S. / Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue. In: Shock. 2015 ; Vol. 44, No. 3. pp. 258-264.
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