Shear stress regulates cystathionine γ lyase expression to preserve endothelial redox balance and reduce membrane lipid peroxidation: Regulation of CSE by KLF2 and miR-27b

Sofia Iris Bibli, Jiong Hu, Matthias S. Leisegang, Janina Wittig, Sven Zukunft, Andrea Kapasakalidi, Beate Fisslthaler, Diamantis Tsilimigras, Georgios Zografos, Konstantinos Filis, Ralf P. Brandes, Andreas Papapetropoulos, Fragiska Sigala, Ingrid Fleming

Research output: Contribution to journalArticle

Abstract

Cystathionine γ lyase (CSE) is the major source of hydrogen sulfide-derived species (H2Sn) in endothelial cells and plays an important role in protecting against atherosclerosis. Here we investigated the molecular mechanisms underlying the regulation of CSE expression in endothelial cells by fluid shear stress/flow. Fluid shear stress decreased CSE expression in human and murine endothelial cells and was negatively correlated with the transcription factor Krüppel-like factor (KLF) 2. CSE was identified as a direct target of the KLF2-regulated microRNA, miR-27b and high expression of CSE in native human plaque-derived endothelial cells, was also inversely correlated with KLF2 and miR-27b levels. One consequence of decreased CSE expression was the loss of Prx6 sulfhydration (on Cys47), which resulted in Prx6 hyperoxidation, decamerization and inhibition, as well as a concomitant increase in endothelial cell reactive oxygen species and lipid membrane peroxidation. H2Sn supplementation in vitro was able to reverse the redox state of Prx6. Statin therapy, which is known to activate KLF2, also decreased CSE expression but increased CSE activity by preventing its phosphorylation on Ser377. As a result, the sulfhydration of Prx6 was partially restored in samples from plaque containing arteries from statin-treated donors. Taken together, the regulation of CSE expression by shear stress/disturbed flow is dependent on KLF2 and miR-27b. Moreover, in murine and human arteries CSE acts to maintain endothelial redox balance at least partly by targeting Prx6 to prevent its decamerization and inhibition of its peroxidase activity.

Original languageEnglish (US)
Article number101379
JournalRedox Biology
Volume28
DOIs
StatePublished - Jan 2020
Externally publishedYes

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Cystathionine
Lyases
Membrane Lipids
Lipid Peroxidation
Oxidation-Reduction
Shear stress
Endothelial cells
Endothelial Cells
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Arteries
Hydrogen Sulfide
Phosphorylation
Fluids
MicroRNAs
Population Groups
Peroxidase
Reactive Oxygen Species
Atherosclerosis
Transcription Factors

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

Cite this

Shear stress regulates cystathionine γ lyase expression to preserve endothelial redox balance and reduce membrane lipid peroxidation : Regulation of CSE by KLF2 and miR-27b. / Bibli, Sofia Iris; Hu, Jiong; Leisegang, Matthias S.; Wittig, Janina; Zukunft, Sven; Kapasakalidi, Andrea; Fisslthaler, Beate; Tsilimigras, Diamantis; Zografos, Georgios; Filis, Konstantinos; Brandes, Ralf P.; Papapetropoulos, Andreas; Sigala, Fragiska; Fleming, Ingrid.

In: Redox Biology, Vol. 28, 101379, 01.2020.

Research output: Contribution to journalArticle

Bibli, SI, Hu, J, Leisegang, MS, Wittig, J, Zukunft, S, Kapasakalidi, A, Fisslthaler, B, Tsilimigras, D, Zografos, G, Filis, K, Brandes, RP, Papapetropoulos, A, Sigala, F & Fleming, I 2020, 'Shear stress regulates cystathionine γ lyase expression to preserve endothelial redox balance and reduce membrane lipid peroxidation: Regulation of CSE by KLF2 and miR-27b', Redox Biology, vol. 28, 101379. https://doi.org/10.1016/j.redox.2019.101379
Bibli, Sofia Iris ; Hu, Jiong ; Leisegang, Matthias S. ; Wittig, Janina ; Zukunft, Sven ; Kapasakalidi, Andrea ; Fisslthaler, Beate ; Tsilimigras, Diamantis ; Zografos, Georgios ; Filis, Konstantinos ; Brandes, Ralf P. ; Papapetropoulos, Andreas ; Sigala, Fragiska ; Fleming, Ingrid. / Shear stress regulates cystathionine γ lyase expression to preserve endothelial redox balance and reduce membrane lipid peroxidation : Regulation of CSE by KLF2 and miR-27b. In: Redox Biology. 2020 ; Vol. 28.
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abstract = "Cystathionine γ lyase (CSE) is the major source of hydrogen sulfide-derived species (H2Sn) in endothelial cells and plays an important role in protecting against atherosclerosis. Here we investigated the molecular mechanisms underlying the regulation of CSE expression in endothelial cells by fluid shear stress/flow. Fluid shear stress decreased CSE expression in human and murine endothelial cells and was negatively correlated with the transcription factor Kr{\"u}ppel-like factor (KLF) 2. CSE was identified as a direct target of the KLF2-regulated microRNA, miR-27b and high expression of CSE in native human plaque-derived endothelial cells, was also inversely correlated with KLF2 and miR-27b levels. One consequence of decreased CSE expression was the loss of Prx6 sulfhydration (on Cys47), which resulted in Prx6 hyperoxidation, decamerization and inhibition, as well as a concomitant increase in endothelial cell reactive oxygen species and lipid membrane peroxidation. H2Sn supplementation in vitro was able to reverse the redox state of Prx6. Statin therapy, which is known to activate KLF2, also decreased CSE expression but increased CSE activity by preventing its phosphorylation on Ser377. As a result, the sulfhydration of Prx6 was partially restored in samples from plaque containing arteries from statin-treated donors. Taken together, the regulation of CSE expression by shear stress/disturbed flow is dependent on KLF2 and miR-27b. Moreover, in murine and human arteries CSE acts to maintain endothelial redox balance at least partly by targeting Prx6 to prevent its decamerization and inhibition of its peroxidase activity.",
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T2 - Regulation of CSE by KLF2 and miR-27b

AU - Bibli, Sofia Iris

AU - Hu, Jiong

AU - Leisegang, Matthias S.

AU - Wittig, Janina

AU - Zukunft, Sven

AU - Kapasakalidi, Andrea

AU - Fisslthaler, Beate

AU - Tsilimigras, Diamantis

AU - Zografos, Georgios

AU - Filis, Konstantinos

AU - Brandes, Ralf P.

AU - Papapetropoulos, Andreas

AU - Sigala, Fragiska

AU - Fleming, Ingrid

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N2 - Cystathionine γ lyase (CSE) is the major source of hydrogen sulfide-derived species (H2Sn) in endothelial cells and plays an important role in protecting against atherosclerosis. Here we investigated the molecular mechanisms underlying the regulation of CSE expression in endothelial cells by fluid shear stress/flow. Fluid shear stress decreased CSE expression in human and murine endothelial cells and was negatively correlated with the transcription factor Krüppel-like factor (KLF) 2. CSE was identified as a direct target of the KLF2-regulated microRNA, miR-27b and high expression of CSE in native human plaque-derived endothelial cells, was also inversely correlated with KLF2 and miR-27b levels. One consequence of decreased CSE expression was the loss of Prx6 sulfhydration (on Cys47), which resulted in Prx6 hyperoxidation, decamerization and inhibition, as well as a concomitant increase in endothelial cell reactive oxygen species and lipid membrane peroxidation. H2Sn supplementation in vitro was able to reverse the redox state of Prx6. Statin therapy, which is known to activate KLF2, also decreased CSE expression but increased CSE activity by preventing its phosphorylation on Ser377. As a result, the sulfhydration of Prx6 was partially restored in samples from plaque containing arteries from statin-treated donors. Taken together, the regulation of CSE expression by shear stress/disturbed flow is dependent on KLF2 and miR-27b. Moreover, in murine and human arteries CSE acts to maintain endothelial redox balance at least partly by targeting Prx6 to prevent its decamerization and inhibition of its peroxidase activity.

AB - Cystathionine γ lyase (CSE) is the major source of hydrogen sulfide-derived species (H2Sn) in endothelial cells and plays an important role in protecting against atherosclerosis. Here we investigated the molecular mechanisms underlying the regulation of CSE expression in endothelial cells by fluid shear stress/flow. Fluid shear stress decreased CSE expression in human and murine endothelial cells and was negatively correlated with the transcription factor Krüppel-like factor (KLF) 2. CSE was identified as a direct target of the KLF2-regulated microRNA, miR-27b and high expression of CSE in native human plaque-derived endothelial cells, was also inversely correlated with KLF2 and miR-27b levels. One consequence of decreased CSE expression was the loss of Prx6 sulfhydration (on Cys47), which resulted in Prx6 hyperoxidation, decamerization and inhibition, as well as a concomitant increase in endothelial cell reactive oxygen species and lipid membrane peroxidation. H2Sn supplementation in vitro was able to reverse the redox state of Prx6. Statin therapy, which is known to activate KLF2, also decreased CSE expression but increased CSE activity by preventing its phosphorylation on Ser377. As a result, the sulfhydration of Prx6 was partially restored in samples from plaque containing arteries from statin-treated donors. Taken together, the regulation of CSE expression by shear stress/disturbed flow is dependent on KLF2 and miR-27b. Moreover, in murine and human arteries CSE acts to maintain endothelial redox balance at least partly by targeting Prx6 to prevent its decamerization and inhibition of its peroxidase activity.

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