Sheep primary cells as in vitro models to investigate Mycoplasma agalactiae host cell interactions

Shrilakshmi Hegde, Cordula Gabriel, Martin Kragl, Rohini Chopra-Dewasthaly

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Appropriate infection models are imperative for the understanding of pathogens like mycoplasmas that are known for their strict host and tissue specificity, and lack of suitable cell and small animal models has hindered pathogenicity studies. This is particularly true for the economically important group of ruminant mycoplasmas whose virulence factors need to be elucidated for designing effective intervention strategies. Mycoplasma agalactiae serves as a useful role model especially because it is phylogenetically very close to M. bovis and causes similar symptoms by as yet unknown mechanisms. Here, we successfully prepared and characterized four different primary sheep cell lines, namely the epithelial and stromal cells from the mammary gland and uterus, respectively. Using immunohistochemistry, we identified vimentin and cytokeratin as specific markers to confirm the typical cell phenotypes of these primary cells. Furthermore, M. agalactiae's consistent adhesion and invasion into these primary cells proves the reliability of these cell models. Mimicking natural infections, mammary epithelial and stromal cells showed higher invasion and adhesion rates compared to the uterine cells as also seen via double immunofluorescence staining. Altogether, we have generated promising in vitro cell models to study host-pathogen interactions of M. agalactiae and related ruminant pathogens in a more authentic manner.

Original languageEnglish (US)
JournalPathogens and Disease
Volume73
Issue number7
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Fingerprint

Mycoplasma agalactiae
Cell Communication
Sheep
Mycoplasma
Ruminants
Stromal Cells
Epithelial Cells
Host-Pathogen Interactions
Organ Specificity
Host Specificity
Virulence Factors
Vimentin
Human Mammary Glands
Keratins
Infection
In Vitro Techniques
Uterus
Fluorescent Antibody Technique
Virulence
Breast

Keywords

  • adhesion
  • host cell interactions
  • immunohistochemistry
  • invasion
  • primary cell culture
  • ruminant mycoplasmosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Sheep primary cells as in vitro models to investigate Mycoplasma agalactiae host cell interactions. / Hegde, Shrilakshmi; Gabriel, Cordula; Kragl, Martin; Chopra-Dewasthaly, Rohini.

In: Pathogens and Disease, Vol. 73, No. 7, 01.10.2015.

Research output: Contribution to journalArticle

Hegde, Shrilakshmi ; Gabriel, Cordula ; Kragl, Martin ; Chopra-Dewasthaly, Rohini. / Sheep primary cells as in vitro models to investigate Mycoplasma agalactiae host cell interactions. In: Pathogens and Disease. 2015 ; Vol. 73, No. 7.
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