Shigella flexneri invasion of HeLa cells induces NF-κB DNA-binding activity

R. B. Dyer, C. R. Collaco, David Niesel, N. K. Herzog

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Although information about the genetic basis and mechanisms of Shigella flexneri cellular invasion is accumulating, little is known about changes in cell signaling and their consequences following bacterium-host cell interactions. A general result of signal transduction is alterations in the levels and/or activities of transcription factors. Alterations in transcription factor binding activities were observed following challenge with S. flexneri. Changes in the DNA-binding activities of cellular transcription factors to AP1, AP2, cyclic AMP response element, CTF1/NF1, NF- κB/Rel, OCT1, and SP1 DNA-binding sites were investigated by electrophoretic mobility shift assays. NF-κB/Rel DNA-binding activity was enhanced more than 11-fold by cellular invasion; noninvasive S. flexneri strains induced low levels of κB DNA binding. Both subunits of the NF-κB transcription factor, p50 and p65, but not c-Rel (p85), are components of the κB DNA-binding activity. These data suggest that changes in cellular transcription factor binding activity are a consequence of S. flexneri invasion, and these changes could play a role in the initial host response or in the pathogenesis of the disease.

Original languageEnglish (US)
Pages (from-to)4427-4433
Number of pages7
JournalInfection and Immunity
Volume61
Issue number10
StatePublished - 1993

Fingerprint

Shigella flexneri
HeLa Cells
Transcription Factors
DNA
Transcription Factor RelA
Response Elements
Electrophoretic Mobility Shift Assay
Cell Communication
Cyclic AMP
Signal Transduction
Binding Sites
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Dyer, R. B., Collaco, C. R., Niesel, D., & Herzog, N. K. (1993). Shigella flexneri invasion of HeLa cells induces NF-κB DNA-binding activity. Infection and Immunity, 61(10), 4427-4433.

Shigella flexneri invasion of HeLa cells induces NF-κB DNA-binding activity. / Dyer, R. B.; Collaco, C. R.; Niesel, David; Herzog, N. K.

In: Infection and Immunity, Vol. 61, No. 10, 1993, p. 4427-4433.

Research output: Contribution to journalArticle

Dyer, RB, Collaco, CR, Niesel, D & Herzog, NK 1993, 'Shigella flexneri invasion of HeLa cells induces NF-κB DNA-binding activity', Infection and Immunity, vol. 61, no. 10, pp. 4427-4433.
Dyer, R. B. ; Collaco, C. R. ; Niesel, David ; Herzog, N. K. / Shigella flexneri invasion of HeLa cells induces NF-κB DNA-binding activity. In: Infection and Immunity. 1993 ; Vol. 61, No. 10. pp. 4427-4433.
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