Short report: Comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus

Dana L. Vanlandingham, Charles E. McGee, Kimberly A. Klingler, Sareen E. Galbraith, Alan Barrett, Stephen Higgs

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phylogenese analysis of West Nile virus in North America has identified replacement of the originally introduced clade, Eastern United States (NY99), by the North American clade. In addition, the transient emergence of other clades and genetic variants has also been observed. In this study, we investigated the potential role of the mosquito in the selection of these clades and genetic variants. We determined the relative susceptibility of Culex quinquefasciatus to infection with isolates from the Eastern U.S. clade, the North American clade, and the Southeast coastal Texas clade. Although significant differences were observed in 50% oral infectious dose values between the Eastern U.S. and two attenuated North American genetic variants compared with the North American and Southeast coastal Texas clade viruses, these differences did not correlate with persistence of the genotype in nature. These results indicate that selection of these viral genotypes was independent of viral oral infectivity in the mosquito.

Original languageEnglish (US)
Pages (from-to)951-954
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number6
StatePublished - Dec 2008

Fingerprint

West Nile virus
Culex
Culicidae
Genotype
North America
Viruses
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Short report : Comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus. / Vanlandingham, Dana L.; McGee, Charles E.; Klingler, Kimberly A.; Galbraith, Sareen E.; Barrett, Alan; Higgs, Stephen.

In: American Journal of Tropical Medicine and Hygiene, Vol. 79, No. 6, 12.2008, p. 951-954.

Research output: Contribution to journalArticle

Vanlandingham, Dana L. ; McGee, Charles E. ; Klingler, Kimberly A. ; Galbraith, Sareen E. ; Barrett, Alan ; Higgs, Stephen. / Short report : Comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 79, No. 6. pp. 951-954.
@article{c51abcdc098047e2901e29efd3582758,
title = "Short report: Comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus",
abstract = "Phylogenese analysis of West Nile virus in North America has identified replacement of the originally introduced clade, Eastern United States (NY99), by the North American clade. In addition, the transient emergence of other clades and genetic variants has also been observed. In this study, we investigated the potential role of the mosquito in the selection of these clades and genetic variants. We determined the relative susceptibility of Culex quinquefasciatus to infection with isolates from the Eastern U.S. clade, the North American clade, and the Southeast coastal Texas clade. Although significant differences were observed in 50{\%} oral infectious dose values between the Eastern U.S. and two attenuated North American genetic variants compared with the North American and Southeast coastal Texas clade viruses, these differences did not correlate with persistence of the genotype in nature. These results indicate that selection of these viral genotypes was independent of viral oral infectivity in the mosquito.",
author = "Vanlandingham, {Dana L.} and McGee, {Charles E.} and Klingler, {Kimberly A.} and Galbraith, {Sareen E.} and Alan Barrett and Stephen Higgs",
year = "2008",
month = "12",
language = "English (US)",
volume = "79",
pages = "951--954",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "6",

}

TY - JOUR

T1 - Short report

T2 - Comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus

AU - Vanlandingham, Dana L.

AU - McGee, Charles E.

AU - Klingler, Kimberly A.

AU - Galbraith, Sareen E.

AU - Barrett, Alan

AU - Higgs, Stephen

PY - 2008/12

Y1 - 2008/12

N2 - Phylogenese analysis of West Nile virus in North America has identified replacement of the originally introduced clade, Eastern United States (NY99), by the North American clade. In addition, the transient emergence of other clades and genetic variants has also been observed. In this study, we investigated the potential role of the mosquito in the selection of these clades and genetic variants. We determined the relative susceptibility of Culex quinquefasciatus to infection with isolates from the Eastern U.S. clade, the North American clade, and the Southeast coastal Texas clade. Although significant differences were observed in 50% oral infectious dose values between the Eastern U.S. and two attenuated North American genetic variants compared with the North American and Southeast coastal Texas clade viruses, these differences did not correlate with persistence of the genotype in nature. These results indicate that selection of these viral genotypes was independent of viral oral infectivity in the mosquito.

AB - Phylogenese analysis of West Nile virus in North America has identified replacement of the originally introduced clade, Eastern United States (NY99), by the North American clade. In addition, the transient emergence of other clades and genetic variants has also been observed. In this study, we investigated the potential role of the mosquito in the selection of these clades and genetic variants. We determined the relative susceptibility of Culex quinquefasciatus to infection with isolates from the Eastern U.S. clade, the North American clade, and the Southeast coastal Texas clade. Although significant differences were observed in 50% oral infectious dose values between the Eastern U.S. and two attenuated North American genetic variants compared with the North American and Southeast coastal Texas clade viruses, these differences did not correlate with persistence of the genotype in nature. These results indicate that selection of these viral genotypes was independent of viral oral infectivity in the mosquito.

UR - http://www.scopus.com/inward/record.url?scp=57649180803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649180803&partnerID=8YFLogxK

M3 - Article

C2 - 19052310

AN - SCOPUS:57649180803

VL - 79

SP - 951

EP - 954

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 6

ER -