Short report: Infection dynamics of sylvatic dengue virus in a natural primate host, the African green monkey

Kathryn A. Hanley, Mathilde Guerbois, Tiffany F. Kautz, Meredith Brown, Stephen S. Whitehead, Scott Weaver, Nikos Vasilakis, Preston A. Marx

Research output: Contribution to journalArticle

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Abstract

The four serotypes of mosquito-borne dengue virus (DENV-1, -2, -3, and -4) that circulate in humans each emerged from an enzootic, sylvatic cycle in non-human primates. Herein, we present the first study of sylvatic DENV infection dynamics in a primate. Three African green monkeys were inoculated with 105plaque-forming units (pfu) DENV-2 strain PM33974 from the sylvatic cycle, and one African green monkey was inoculated with 105pfu DENV-2 strain New Guinea C from the human cycle. All four monkeys seroconverted (more than fourfold rise in 80% plaque reduction neutralization titer [PRNT80]) against the strain of DENV with which they were inoculated; only one (33%) of three monkeys infected with sylvatic DENV showed a neutralizing antibody response against human-endemic DENV. Virus was detected in two of three monkeys inoculated with sylvatic DENV at low titer (≤ 1.3 log10pfu/mL) and brief duration (≤ 2 days). Clinical signs included rash and elevated aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels.

Original languageEnglish (US)
Pages (from-to)672-676
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume91
Issue number4
DOIs
StatePublished - Oct 1 2014

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Cercopithecus aethiops
Dengue Virus
Primates
Haplorhini
Infection
New Guinea
Aspartate Aminotransferases
Exanthema
Neutralizing Antibodies
Culicidae
Alanine Transaminase
Antibody Formation
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Short report : Infection dynamics of sylvatic dengue virus in a natural primate host, the African green monkey. / Hanley, Kathryn A.; Guerbois, Mathilde; Kautz, Tiffany F.; Brown, Meredith; Whitehead, Stephen S.; Weaver, Scott; Vasilakis, Nikos; Marx, Preston A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 91, No. 4, 01.10.2014, p. 672-676.

Research output: Contribution to journalArticle

Hanley, Kathryn A. ; Guerbois, Mathilde ; Kautz, Tiffany F. ; Brown, Meredith ; Whitehead, Stephen S. ; Weaver, Scott ; Vasilakis, Nikos ; Marx, Preston A. / Short report : Infection dynamics of sylvatic dengue virus in a natural primate host, the African green monkey. In: American Journal of Tropical Medicine and Hygiene. 2014 ; Vol. 91, No. 4. pp. 672-676.
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