TY - JOUR
T1 - Short report
T2 - Isolation and characterization of sylvatic mosquito-borne viruses in Trinidad: Enzootic transmission and a new potential vector of Mucambo virus
AU - Auguste, Albert J.
AU - Adams, A. Paige
AU - Arrigo, Nicole C.
AU - Martinez, Raymond
AU - Travassos Da Rosa, Amelia P.A.
AU - Adesiyun, Abiodun A.
AU - Chadee, Dave D.
AU - Tesh, Robert B.
AU - Carrington, Christine V.F.
AU - Weaver, Scott C.
PY - 2010/12
Y1 - 2010/12
N2 - Mosquito surveillance was carried out in three forested regions of Trinidad during July 2007-March 2009. A total of 185,397 mosquitoes representing at least 46 species was collected, divided into pools of 1-50 mosquitoes according to species and sex, and screened for arboviruses using cytopathic effect assays on Vero cell monolayers. Eighty-five viruses were isolated, including members of the genera Alphavirus (Mucambo virus; MUCV) and Orthobunyavirus (Caraparu, Oriboca, Bimiti, and Wyeomyia viruses). Species of the Culex subgenus Melanoconion accounted for 56% of the total number of mosquitoes collected and 97% of the viruses isolated; Cx. (Mel.) portesi accounted for 92% of virus isolations. Our results also implicate for the first time Aedes (Ochlerotatus) hortator as a potential vector of MUCV. Phylogenetic analyses of 43 MUCV strains suggest population subdivision within Trinidad, consistent with the hypothesis of enzootic maintenance in localized rodent populations.
AB - Mosquito surveillance was carried out in three forested regions of Trinidad during July 2007-March 2009. A total of 185,397 mosquitoes representing at least 46 species was collected, divided into pools of 1-50 mosquitoes according to species and sex, and screened for arboviruses using cytopathic effect assays on Vero cell monolayers. Eighty-five viruses were isolated, including members of the genera Alphavirus (Mucambo virus; MUCV) and Orthobunyavirus (Caraparu, Oriboca, Bimiti, and Wyeomyia viruses). Species of the Culex subgenus Melanoconion accounted for 56% of the total number of mosquitoes collected and 97% of the viruses isolated; Cx. (Mel.) portesi accounted for 92% of virus isolations. Our results also implicate for the first time Aedes (Ochlerotatus) hortator as a potential vector of MUCV. Phylogenetic analyses of 43 MUCV strains suggest population subdivision within Trinidad, consistent with the hypothesis of enzootic maintenance in localized rodent populations.
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U2 - 10.4269/ajtmh.2010.10-0280
DO - 10.4269/ajtmh.2010.10-0280
M3 - Article
C2 - 21118932
AN - SCOPUS:79551654051
SN - 0002-9637
VL - 83
SP - 1262
EP - 1265
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 6
ER -