Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model: Effects on microcirculation and antioxidant status in rats

Attila Szijártó, Oszkár Hahn, Enkhjargal Batmunkh, Rita Stangl, András Kiss, Gábor Lotz, Zsuzsa Schaff, László Váli, Anna Blázovics, Domokos Gero, Csaba Szabo, Péter Kupcsulik, László Harsányi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.

Original languageEnglish (US)
Pages (from-to)640-648
Number of pages9
JournalClinical Nutrition
Volume26
Issue number5
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

alanylglutamine
Dipeptides
Microcirculation
Reperfusion
Ischemia
Antioxidants
Glutamine
Liver
Reperfusion Injury
Caspase 3
Serum
Laser-Doppler Flowmetry
Poisons
In Situ Nick-End Labeling
Free Radicals

Keywords

  • Apoptosis
  • Glutamine
  • Ischemia
  • Laser Doppler flowmeter
  • Liver
  • Reperfusion

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Endocrinology, Diabetes and Metabolism
  • Gastroenterology
  • Health Professions(all)
  • Medicine (miscellaneous)

Cite this

Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model : Effects on microcirculation and antioxidant status in rats. / Szijártó, Attila; Hahn, Oszkár; Batmunkh, Enkhjargal; Stangl, Rita; Kiss, András; Lotz, Gábor; Schaff, Zsuzsa; Váli, László; Blázovics, Anna; Gero, Domokos; Szabo, Csaba; Kupcsulik, Péter; Harsányi, László.

In: Clinical Nutrition, Vol. 26, No. 5, 10.2007, p. 640-648.

Research output: Contribution to journalArticle

Szijártó, A, Hahn, O, Batmunkh, E, Stangl, R, Kiss, A, Lotz, G, Schaff, Z, Váli, L, Blázovics, A, Gero, D, Szabo, C, Kupcsulik, P & Harsányi, L 2007, 'Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model: Effects on microcirculation and antioxidant status in rats', Clinical Nutrition, vol. 26, no. 5, pp. 640-648. https://doi.org/10.1016/j.clnu.2007.06.011
Szijártó, Attila ; Hahn, Oszkár ; Batmunkh, Enkhjargal ; Stangl, Rita ; Kiss, András ; Lotz, Gábor ; Schaff, Zsuzsa ; Váli, László ; Blázovics, Anna ; Gero, Domokos ; Szabo, Csaba ; Kupcsulik, Péter ; Harsányi, László. / Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model : Effects on microcirculation and antioxidant status in rats. In: Clinical Nutrition. 2007 ; Vol. 26, No. 5. pp. 640-648.
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abstract = "Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.",
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T1 - Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model

T2 - Effects on microcirculation and antioxidant status in rats

AU - Szijártó, Attila

AU - Hahn, Oszkár

AU - Batmunkh, Enkhjargal

AU - Stangl, Rita

AU - Kiss, András

AU - Lotz, Gábor

AU - Schaff, Zsuzsa

AU - Váli, László

AU - Blázovics, Anna

AU - Gero, Domokos

AU - Szabo, Csaba

AU - Kupcsulik, Péter

AU - Harsányi, László

PY - 2007/10

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N2 - Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.

AB - Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.

KW - Apoptosis

KW - Glutamine

KW - Ischemia

KW - Laser Doppler flowmeter

KW - Liver

KW - Reperfusion

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