TY - JOUR
T1 - Short-term alanyl-glutamine dipeptide pretreatment in liver ischemia-reperfusion model
T2 - Effects on microcirculation and antioxidant status in rats
AU - Szijártó, Attila
AU - Hahn, Oszkár
AU - Batmunkh, Enkhjargal
AU - Stangl, Rita
AU - Kiss, András
AU - Lotz, Gábor
AU - Schaff, Zsuzsa
AU - Váli, László
AU - Blázovics, Anna
AU - Gero, Domokos
AU - Szabó, Csaba
AU - Kupcsulik, Péter
AU - Harsányi, László
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/10
Y1 - 2007/10
N2 - Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.
AB - Background & aims: Ischemia-reperfusion (I-R) injury is responsible for the morbidity associated with liver surgery. Production of toxic free radicals influences the microcirculation. The aim of our study was to examine the effect of glutamine (Gln) supplementation-adminstered in alanyl-glutamine dipeptide form-on liver function, immuno/histopathology and the oxidative state of the liver after injury. Methods: Two-hundred and fifty grams male Wistar rats underwent normothermic, 60 min, segmental liver ischemia followed by 6 h of reperfusion. The animals (n=45) were divided into three groups: sham operated, I-R and parenteral Gln pretreatment. Hepatic microcirculation was monitored by laser Doppler flowmetry. At the 6 h of reperfusion, histological alterations, TUNEL reaction, active caspase-3 reaction, serum and liver tissue antioxidant levels, serum ALAT, ASAT and TNF-α levels were measured. Results: Upon reperfusion, the Gln group had significantly (p<0.05) higher flow rates than the I-R group and, at the end of the 6 h of reperfusion, significantly (p<0.05) lower serum ALAT and ASAT levels. The liver chemiluminescent intensity was lower, free SH-groups were elevated, while the reducing power was decreased in the Gln-pretreated group. Positive staining for caspase-3 after Gln pretreatment was significantly increased in contrast to the control tissues. Conclusion: Glutamine pretreatment is beneficial in supporting hepatic microcirculation and can prevent hepatocellular necrosis in liver reperfusion injury.
KW - Apoptosis
KW - Glutamine
KW - Ischemia
KW - Laser Doppler flowmeter
KW - Liver
KW - Reperfusion
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U2 - 10.1016/j.clnu.2007.06.011
DO - 10.1016/j.clnu.2007.06.011
M3 - Article
C2 - 17689840
AN - SCOPUS:35448987283
SN - 0261-5614
VL - 26
SP - 640
EP - 648
JO - Clinical Nutrition
JF - Clinical Nutrition
IS - 5
ER -