Short-term oxandrolone administration stimulates net muscle protein synthesis in young men

Melinda Sheffield-Moore, Randall Urban, Steven Wolf, J. Jiang, Don H. Catlin, David Herndon, Robert R. Wolfe, Arny A. Ferrando

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Short term administration of testosterone stimulates net protein synthesis in healthy men. We investigated whether oxandrolone [Oxandrin (OX)], a synthetic analog of testosterone, would improve net muscle protein synthesis and transport of amino acids across the leg. Six healthy men [22 ± 1 (±SE) yr] were studied in the postabsorptive state before and after 5 days of oral OX (15 mg/day). Muscle protein synthesis and breakdown were determined by a three-compartment model using stable isotopic data obtained from femoral arterio-venous sampling and muscle biopsy. The precursor-product method was used to determine muscle protein fractional synthetic rates. Fractional breakdown rates were also directly calculated. Total messenger ribonucleic acid (mRNA) concentrations of skeletal muscle insulin-like growth factor I and androgen receptor (AR) were determined using RT-PCR. Model-derived muscle protein synthesis increased from 53.5 ± 3 to 68.3 ± 5 (mean ± SE) nmol/min·100 mL/leg (P < 0.05), whereas protein breakdown was unchanged. Inward transport of amino acids remained unchanged with OX, whereas outward transport decreased (P < 0.05). The fractional synthetic rate increased 44% (P < 0.05) after OX administration, with no change in fractional breakdown rate. Therefore, the net balance between synthesis and breakdown became more positive with both methodologies (P < 0.05) and was not different from zero. Further, RT-PCR showed that OX administration significantly increased mRNA concentrations of skeletal muscle AR without changing insulin-like growth factor I mRNA concentrations. We conclude that short term OX administration stimulated an increase in skeletal muscle protein synthesis and improved intracellular reutilization of amino acids. The mechanism for this stimulation may be related to an OX-induced increase in AR expression in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)2705-2711
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number8
StatePublished - 1999

Fingerprint

Oxandrolone
Muscle Proteins
Muscle
Androgen Receptors
Skeletal Muscle
RNA
Amino Acids
Testosterone
Leg
Polymerase Chain Reaction
IGF Type 1 Receptor
Biopsy
Protein Transport
Thigh
Insulin-Like Growth Factor I
Proteins
Sampling

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Sheffield-Moore, M., Urban, R., Wolf, S., Jiang, J., Catlin, D. H., Herndon, D., ... Ferrando, A. A. (1999). Short-term oxandrolone administration stimulates net muscle protein synthesis in young men. Journal of Clinical Endocrinology and Metabolism, 84(8), 2705-2711.

Short-term oxandrolone administration stimulates net muscle protein synthesis in young men. / Sheffield-Moore, Melinda; Urban, Randall; Wolf, Steven; Jiang, J.; Catlin, Don H.; Herndon, David; Wolfe, Robert R.; Ferrando, Arny A.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 8, 1999, p. 2705-2711.

Research output: Contribution to journalArticle

Sheffield-Moore, M, Urban, R, Wolf, S, Jiang, J, Catlin, DH, Herndon, D, Wolfe, RR & Ferrando, AA 1999, 'Short-term oxandrolone administration stimulates net muscle protein synthesis in young men', Journal of Clinical Endocrinology and Metabolism, vol. 84, no. 8, pp. 2705-2711.
Sheffield-Moore, Melinda ; Urban, Randall ; Wolf, Steven ; Jiang, J. ; Catlin, Don H. ; Herndon, David ; Wolfe, Robert R. ; Ferrando, Arny A. / Short-term oxandrolone administration stimulates net muscle protein synthesis in young men. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 8. pp. 2705-2711.
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