Signal peptides leading secretory expression of foreign genes in insect expression system

Ping Ji, Xin Min Zha, Guo Ying Zhang, Wei De Shen

Research output: Contribution to journalArticle

Abstract

Fusing nucleotde sequences of signal peptides of the arrowhead proeinase inhibitor of plant origin and gp67 of baculoirus origin with the arrowhead proteinase inhibitor (API) gene and colning them into insect baculovirus transfer vector pBacPAK8 respectively, then integrating each into insect virus expression vector Bm - BacPAK6, we harvested recombinant virus of CrBK - API4 and CrBK - gpAPI2. Inhibitor was high - level expressed. Exogenous expression products almost existed in supernatant fluid of extracellular region in Bm cells; expression in larvae showed that the signal peptides can lead foreign genes to express efficiently and 90Percent of the expression products can secret to extracellular region;in pupae, the situation was similar to sarae, but expressing products appeared to be heterogeneous, compared with larvae, there is a surplus inferior band with molecular weight about 1KD in pupae, From molecular weight of the expressing products by SDS - PAGE, we can infer that the two signal peptides were likely not cut in the expression procedure.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalJournal of Soochow University Engineering Science Edition
Volume23
Issue number2
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Insect Genes
Sagittaria
Protein Sorting Signals
signal peptide
Pupa
Genes
Viruses
Gene Expression
insects
Larva
pupae
Insect Viruses
Molecular Weight
Molecular weight
insect viruses
molecular weight
extracellular fluids
genes
Baculoviridae
larvae

Keywords

  • Insect expression system
  • Proteinase inhibitor
  • Secretory expression
  • Signal peptide

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Agricultural and Biological Sciences(all)
  • Polymers and Plastics

Cite this

Signal peptides leading secretory expression of foreign genes in insect expression system. / Ji, Ping; Zha, Xin Min; Zhang, Guo Ying; Shen, Wei De.

In: Journal of Soochow University Engineering Science Edition, Vol. 23, No. 2, 04.2003, p. 10-16.

Research output: Contribution to journalArticle

@article{bbcb274e07fc4f368a70128b4f6a8605,
title = "Signal peptides leading secretory expression of foreign genes in insect expression system",
abstract = "Fusing nucleotde sequences of signal peptides of the arrowhead proeinase inhibitor of plant origin and gp67 of baculoirus origin with the arrowhead proteinase inhibitor (API) gene and colning them into insect baculovirus transfer vector pBacPAK8 respectively, then integrating each into insect virus expression vector Bm - BacPAK6, we harvested recombinant virus of CrBK - API4 and CrBK - gpAPI2. Inhibitor was high - level expressed. Exogenous expression products almost existed in supernatant fluid of extracellular region in Bm cells; expression in larvae showed that the signal peptides can lead foreign genes to express efficiently and 90Percent of the expression products can secret to extracellular region;in pupae, the situation was similar to sarae, but expressing products appeared to be heterogeneous, compared with larvae, there is a surplus inferior band with molecular weight about 1KD in pupae, From molecular weight of the expressing products by SDS - PAGE, we can infer that the two signal peptides were likely not cut in the expression procedure.",
keywords = "Insect expression system, Proteinase inhibitor, Secretory expression, Signal peptide",
author = "Ping Ji and Zha, {Xin Min} and Zhang, {Guo Ying} and Shen, {Wei De}",
year = "2003",
month = "4",
language = "English (US)",
volume = "23",
pages = "10--16",
journal = "Journal of Soochow University Engineering Science Edition",
issn = "1673-047X",
publisher = "Soochow University Press",
number = "2",

}

TY - JOUR

T1 - Signal peptides leading secretory expression of foreign genes in insect expression system

AU - Ji, Ping

AU - Zha, Xin Min

AU - Zhang, Guo Ying

AU - Shen, Wei De

PY - 2003/4

Y1 - 2003/4

N2 - Fusing nucleotde sequences of signal peptides of the arrowhead proeinase inhibitor of plant origin and gp67 of baculoirus origin with the arrowhead proteinase inhibitor (API) gene and colning them into insect baculovirus transfer vector pBacPAK8 respectively, then integrating each into insect virus expression vector Bm - BacPAK6, we harvested recombinant virus of CrBK - API4 and CrBK - gpAPI2. Inhibitor was high - level expressed. Exogenous expression products almost existed in supernatant fluid of extracellular region in Bm cells; expression in larvae showed that the signal peptides can lead foreign genes to express efficiently and 90Percent of the expression products can secret to extracellular region;in pupae, the situation was similar to sarae, but expressing products appeared to be heterogeneous, compared with larvae, there is a surplus inferior band with molecular weight about 1KD in pupae, From molecular weight of the expressing products by SDS - PAGE, we can infer that the two signal peptides were likely not cut in the expression procedure.

AB - Fusing nucleotde sequences of signal peptides of the arrowhead proeinase inhibitor of plant origin and gp67 of baculoirus origin with the arrowhead proteinase inhibitor (API) gene and colning them into insect baculovirus transfer vector pBacPAK8 respectively, then integrating each into insect virus expression vector Bm - BacPAK6, we harvested recombinant virus of CrBK - API4 and CrBK - gpAPI2. Inhibitor was high - level expressed. Exogenous expression products almost existed in supernatant fluid of extracellular region in Bm cells; expression in larvae showed that the signal peptides can lead foreign genes to express efficiently and 90Percent of the expression products can secret to extracellular region;in pupae, the situation was similar to sarae, but expressing products appeared to be heterogeneous, compared with larvae, there is a surplus inferior band with molecular weight about 1KD in pupae, From molecular weight of the expressing products by SDS - PAGE, we can infer that the two signal peptides were likely not cut in the expression procedure.

KW - Insect expression system

KW - Proteinase inhibitor

KW - Secretory expression

KW - Signal peptide

UR - http://www.scopus.com/inward/record.url?scp=33846402705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846402705&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33846402705

VL - 23

SP - 10

EP - 16

JO - Journal of Soochow University Engineering Science Edition

JF - Journal of Soochow University Engineering Science Edition

SN - 1673-047X

IS - 2

ER -