Significance of lipid-derived reactive aldehyde-specificimmune complexes in systemic lupus erythematosus

Gangduo Wang, Silvia S. Pierangeli, Rohan Willis, Emilio Gonzalez, Michelle Petri, M Khan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Even though systemic lupus erythematosus (SLE) is associated with high morbidity and mortality rates among young and middle-aged women, the molecular mechanisms of disease pathogenesis are not fully understood. Previous studies from our laboratory suggested an association between oxidative stress and SLE disease activity (SLEDAI). To further assess the role of reactive oxygen species (ROS) in SLE, we examined the contribution of lipid-derived reactive aldehydes (LDRAs)-specific immune complexes in SLE. Sera from 60 SLE patients with varying SLEDAI and 32 age- and gender- matched healthy controls were analyzed for oxidative stress and related markers. Patients were divided into two groups based on their SLEDAI scores (<6 and ≥ 6). Both SLEDAI groups showed higher serum 4-hydroxynonenal (HNE)-/malondialdehyde (MDA)-protein adducts and their specific immune complexes (HNE-/MDA-specific ICs) together with IL-17 than the controls, but the levels were significantly greater in the high SLEDAI (≥ 6) group. Moreover, the serum levels of anti-oxidant enzymes Cu/Zn superoxide dismutase (SOD) and catalase (CAT) were significantly reduced in both patient groups compared to controls. Remarkably, for the first time, our data show that increased HNE-/MDA-specific ICs are positively associated with SLEDAI and elevated circulating immune complexes (CICs), suggesting a possible causal relationship among oxidative stress, LDRA-specific ICs and the development of SLE. Our findings, apart from providing firm support to an association between oxidative stress and SLE, also suggest that these oxidative stress markers, especially the HNE-/ MDA-specific ICs, may be useful in evaluating the prognosis of SLE as well as in elucidating the mechanisms of disease pathogenesis.

Original languageEnglish (US)
Article numbere0164739
JournalPLoS One
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

lupus erythematosus
Aldehydes
Systemic Lupus Erythematosus
aldehydes
Oxidative stress
Lipids
lipids
Oxidative Stress
Malondialdehyde
oxidative stress
malondialdehyde
antigen-antibody complex
Antigen-Antibody Complex
pathogenesis
Serum
middle-aged adults
Association reactions
Interleukin-17
Oxidants
oxidants

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Significance of lipid-derived reactive aldehyde-specificimmune complexes in systemic lupus erythematosus. / Wang, Gangduo; Pierangeli, Silvia S.; Willis, Rohan; Gonzalez, Emilio; Petri, Michelle; Khan, M.

In: PLoS One, Vol. 11, No. 10, e0164739, 01.10.2016.

Research output: Contribution to journalArticle

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