Simultaneous determination of myocardial creatine phosphate and adenine nucleotides by reversed‐phase HPLC

Radhika Sanduja, G. A.S. Ansari, Paul J. Boor

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An isocratic HPLC system has been developed which allows for the rapid (single run of 20 min) measurement of creatine phosphate (PCr) and adenine nucleotides (ATP, ADP and AMP) in extracts from freeze‐clamped and freeze‐dried myocardial tissues. The separation was achieved at room temperature by using a RP18 column and a dual variable wavelength spectrophotometer, set at 210 and 254 nm. The solvent was 30 mM potassium dihydrogen phosphate, 15 mM tetrabutylammonium hydrogen sulfate, pH 6.7, 19% (v/v) acetonitrile. A distinct separation (confirmed with the retention time of standard sample) of these high energy compounds was achieved. Standard curves were linear. In isolated rat hearts the following values were obtained (μmol/g dry wt, mean ± SEM): ATP 21.5 ± 1.3, ADP 4.6 ± 0.2, AMP 1.5 ± 1.1 and PCr 32.5 ± 1.3; which are consistent with previously published values for high energy compounds in this tissue.

Original languageEnglish (US)
Pages (from-to)156-158
Number of pages3
JournalBiomedical Chromatography
Volume2
Issue number4
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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