Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography-tandem mass spectrometry

Xiao-Ming Wang, Daria I. Vernikovskaya, Doaa R. Abdelrahman, Gary Hankins, Mahmoud Ahmed, Tatiana Nanovskaya

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19 Citations (Scopus)

Abstract

A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of bupropion and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18 column, with an isocratic elution of 31% methanol and 69% formic acid (0.04%, v/v) aqueous solution at a flow rate of 1.0mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2>0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is <0.72ng/mL and 0.92ng/g in placental tissue samples. The relative deviation of this method was <15% for intra- and inter-day assays, and the accuracy ranged between 88% and 105%. The extraction recovery of the four analytes ranged between 89% and 96% in umbilical cord plasma, and 64% and 80% in placental tissue. No significant matrix effect was observed in the presented method.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume70
DOIs
StatePublished - Nov 2012

Fingerprint

Bupropion
Umbilical Cord
High performance liquid chromatography
Metabolites
Tandem Mass Spectrometry
Mass spectrometry
formic acid
High Pressure Liquid Chromatography
Tissue
Plasmas
Ionization
Mass Spectrometry
Trichloroacetic Acid
Deuterium
Liquid chromatography
Isotopes
Methanol
Assays
Flow rate
Liquid Chromatography

Keywords

  • BUP
  • Bupropion
  • EB
  • ESI
  • LC-MS/MS
  • LLOQ
  • LOD
  • Metabolites
  • MRM
  • MS
  • OH-BUP
  • Placenta
  • S/N
  • TB
  • TCA
  • Umbilical cord plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

@article{afddd964ed9e47c1a9d837161beaaf2b,
title = "Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography-tandem mass spectrometry",
abstract = "A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of bupropion and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18 column, with an isocratic elution of 31{\%} methanol and 69{\%} formic acid (0.04{\%}, v/v) aqueous solution at a flow rate of 1.0mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2>0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is <0.72ng/mL and 0.92ng/g in placental tissue samples. The relative deviation of this method was <15{\%} for intra- and inter-day assays, and the accuracy ranged between 88{\%} and 105{\%}. The extraction recovery of the four analytes ranged between 89{\%} and 96{\%} in umbilical cord plasma, and 64{\%} and 80{\%} in placental tissue. No significant matrix effect was observed in the presented method.",
keywords = "BUP, Bupropion, EB, ESI, LC-MS/MS, LLOQ, LOD, Metabolites, MRM, MS, OH-BUP, Placenta, S/N, TB, TCA, Umbilical cord plasma",
author = "Xiao-Ming Wang and Vernikovskaya, {Daria I.} and Abdelrahman, {Doaa R.} and Gary Hankins and Mahmoud Ahmed and Tatiana Nanovskaya",
year = "2012",
month = "11",
doi = "10.1016/j.jpba.2012.05.008",
language = "English (US)",
volume = "70",
pages = "320--329",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
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TY - JOUR

T1 - Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography-tandem mass spectrometry

AU - Wang, Xiao-Ming

AU - Vernikovskaya, Daria I.

AU - Abdelrahman, Doaa R.

AU - Hankins, Gary

AU - Ahmed, Mahmoud

AU - Nanovskaya, Tatiana

PY - 2012/11

Y1 - 2012/11

N2 - A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of bupropion and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18 column, with an isocratic elution of 31% methanol and 69% formic acid (0.04%, v/v) aqueous solution at a flow rate of 1.0mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2>0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is <0.72ng/mL and 0.92ng/g in placental tissue samples. The relative deviation of this method was <15% for intra- and inter-day assays, and the accuracy ranged between 88% and 105%. The extraction recovery of the four analytes ranged between 89% and 96% in umbilical cord plasma, and 64% and 80% in placental tissue. No significant matrix effect was observed in the presented method.

AB - A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of bupropion and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18 column, with an isocratic elution of 31% methanol and 69% formic acid (0.04%, v/v) aqueous solution at a flow rate of 1.0mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2>0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is <0.72ng/mL and 0.92ng/g in placental tissue samples. The relative deviation of this method was <15% for intra- and inter-day assays, and the accuracy ranged between 88% and 105%. The extraction recovery of the four analytes ranged between 89% and 96% in umbilical cord plasma, and 64% and 80% in placental tissue. No significant matrix effect was observed in the presented method.

KW - BUP

KW - Bupropion

KW - EB

KW - ESI

KW - LC-MS/MS

KW - LLOQ

KW - LOD

KW - Metabolites

KW - MRM

KW - MS

KW - OH-BUP

KW - Placenta

KW - S/N

KW - TB

KW - TCA

KW - Umbilical cord plasma

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U2 - 10.1016/j.jpba.2012.05.008

DO - 10.1016/j.jpba.2012.05.008

M3 - Article

C2 - 22682512

AN - SCOPUS:84866248910

VL - 70

SP - 320

EP - 329

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -