Single-channel recording of TASK-3-like K+ channel and up-regulation of TASK-3 mRNA expression after spinal cord injury in rat dorsal root ganglion neurons

Inseok Jang, Jun-Ho La, Gyu Tae Kim, Jeong Soon Lee, Eun Jin Kim, Eun Shin Lee, Su Jeong Kim, Jeong Min Seo, Sang Ho Ahn, Jae Yong Park, Seong Geun Hong, Dawon Kang, Jaehee Han

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K+ channel was 33.0±0.1 pS at -60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K+ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K+ channel under SCI-induced acidic condition.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Volume12
Issue number5
StatePublished - Oct 2008
Externally publishedYes

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Spinal Ganglia
Spinal Cord Injuries
Up-Regulation
Neurons
Messenger RNA
Spinal Nerve Roots
Spinal Cord
Brain
Proteins

Keywords

  • Acidosis
  • Dorsal root ganglion
  • Spinal cord injuries
  • Two-pore domain K channel

ASJC Scopus subject areas

  • Pharmacology
  • Physiology

Cite this

Single-channel recording of TASK-3-like K+ channel and up-regulation of TASK-3 mRNA expression after spinal cord injury in rat dorsal root ganglion neurons. / Jang, Inseok; La, Jun-Ho; Kim, Gyu Tae; Lee, Jeong Soon; Kim, Eun Jin; Lee, Eun Shin; Kim, Su Jeong; Seo, Jeong Min; Ahn, Sang Ho; Park, Jae Yong; Hong, Seong Geun; Kang, Dawon; Han, Jaehee.

In: Korean Journal of Physiology and Pharmacology, Vol. 12, No. 5, 10.2008, p. 245-251.

Research output: Contribution to journalArticle

Jang, I, La, J-H, Kim, GT, Lee, JS, Kim, EJ, Lee, ES, Kim, SJ, Seo, JM, Ahn, SH, Park, JY, Hong, SG, Kang, D & Han, J 2008, 'Single-channel recording of TASK-3-like K+ channel and up-regulation of TASK-3 mRNA expression after spinal cord injury in rat dorsal root ganglion neurons', Korean Journal of Physiology and Pharmacology, vol. 12, no. 5, pp. 245-251.
Jang, Inseok ; La, Jun-Ho ; Kim, Gyu Tae ; Lee, Jeong Soon ; Kim, Eun Jin ; Lee, Eun Shin ; Kim, Su Jeong ; Seo, Jeong Min ; Ahn, Sang Ho ; Park, Jae Yong ; Hong, Seong Geun ; Kang, Dawon ; Han, Jaehee. / Single-channel recording of TASK-3-like K+ channel and up-regulation of TASK-3 mRNA expression after spinal cord injury in rat dorsal root ganglion neurons. In: Korean Journal of Physiology and Pharmacology. 2008 ; Vol. 12, No. 5. pp. 245-251.
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abstract = "Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K+ channel was 33.0±0.1 pS at -60 mV, and TASK-3 activity fell by 65±5{\%} when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K+ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K+ channel under SCI-induced acidic condition.",
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AU - Lee, Jeong Soon

AU - Kim, Eun Jin

AU - Lee, Eun Shin

AU - Kim, Su Jeong

AU - Seo, Jeong Min

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AU - Hong, Seong Geun

AU - Kang, Dawon

AU - Han, Jaehee

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AB - Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K+ channel was 33.0±0.1 pS at -60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K+ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K+ channel under SCI-induced acidic condition.

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