Single mechanosensitive and Ca2+-sensitive channel currents recorded from mouse and human embryonic stem cells

Bernat Soria, Sergio Navas, Abdelkrim Hmadcha, Owen P. Hamill

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca2+ permeant cation channels (MscCa) and large conductance (200 pS) Ca2+-sensitive K+ (BKCa2+) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BKCa2+ channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca2+-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca2+] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.

Original languageEnglish (US)
Pages (from-to)215-230
Number of pages16
JournalJournal of Membrane Biology
Volume246
Issue number3
DOIs
StatePublished - Mar 1 2013

Keywords

  • Calcium sensitive channels
  • Embryonic stem cells
  • Mechanosensitive channels

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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