Single-molecule force spectroscopy of polycystic kidney disease proteins

Liang Ma, Meixiang Xu, Andres F. Oberhauser

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.

Original languageEnglish (US)
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages14
ISBN (Print)9781617798054
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Atomic force microscopy
  • Mechanical properties
  • Polycystic kidney disease
  • Polycystin
  • Protein elasticity
  • Protein mechanics
  • Single-molecule force spectroscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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