Abstract
Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.
| Original language | English (US) |
|---|---|
| Title of host publication | Spectroscopic Methods of Analysis |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 297-310 |
| Number of pages | 14 |
| ISBN (Print) | 9781617798054 |
| DOIs | |
| State | Published - Jan 1 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 875 |
| ISSN (Print) | 1064-3745 |
Keywords
- Atomic force microscopy
- Mechanical properties
- Polycystic kidney disease
- Polycystin
- Protein elasticity
- Protein mechanics
- Single-molecule force spectroscopy
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Ma, L., Xu, M., & Oberhauser, A. F. (2012). Single-molecule force spectroscopy of polycystic kidney disease proteins. In Spectroscopic Methods of Analysis: Methods and Protocols (pp. 297-310). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_16