Abstract
Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 297-310 |
| Number of pages | 14 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 875 |
| DOIs | |
| State | Published - 2012 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Single-molecule force spectroscopy of polycystic kidney disease proteins. / Ma, Liang; Xu, Meixiang; Oberhauser, Andres.
In: Methods in molecular biology (Clifton, N.J.), Vol. 875, 2012, p. 297-310.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-molecule force spectroscopy of polycystic kidney disease proteins.
AU - Ma, Liang
AU - Xu, Meixiang
AU - Oberhauser, Andres
PY - 2012
Y1 - 2012
N2 - Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.
AB - Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.
UR - http://www.scopus.com/inward/record.url?scp=84865808230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865808230&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-806-1_16
DO - 10.1007/978-1-61779-806-1_16
M3 - Article
VL - 875
SP - 297
EP - 310
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -