Single-molecule force spectroscopy of polycystic kidney disease proteins

Liang Ma, Meixiang Xu, Andres F. Oberhauser

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Scopus citations

    Abstract

    Atomic force microscopy in its single-molecule force spectroscopy mode is a nanomanipulation technique that is extensively used for the study of the mechanical properties of proteins. It is particularly suited to examine their response to stretching (i.e., molecular elasticity and mechanical stability). Here, we describe protein engineering strategies and single-molecule AFM techniques for probing protein mechanics, with special emphasis on polycystic kidney disease (PKD) proteins. We also provide step-by-step protocols for preparing proteins and performing single-molecule force measurements.

    Original languageEnglish (US)
    Title of host publicationSpectroscopic Methods of Analysis
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Pages297-310
    Number of pages14
    ISBN (Print)9781617798054
    DOIs
    StatePublished - 2012

    Publication series

    NameMethods in Molecular Biology
    Volume875
    ISSN (Print)1064-3745

    Keywords

    • Atomic force microscopy
    • Mechanical properties
    • Polycystic kidney disease
    • Polycystin
    • Protein elasticity
    • Protein mechanics
    • Single-molecule force spectroscopy

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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