Single-molecule methods

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Single-molecule methods have emerged as powerful tools in life science research. These techniques allow the detection and manipulation of individual biological molecules and investigate, with unprecedented resolution, their conformations and dynamics at the nanoscale level. These techniques overcome the restrictions of traditional bulk biochemical studies by focusing on individuals of molecules. Here we describe some of the most common single-molecule methods including atomic force microscopy, optical tweezers, and fluorescence microscopy. We also describe the use of cryo-electron microscopy methods to study large molecules and macromolecular assemblies. We outline the principles of operation for each technique and discuss prominent applications.

Original languageEnglish (US)
Title of host publicationMolecular Biophysics for the Life Sciences
PublisherSpringer New York
Pages257-288
Number of pages32
ISBN (Electronic)9781461485483
ISBN (Print)9781461485476
DOIs
StatePublished - Jan 1 2013

Fingerprint

Molecules
Optical Tweezers
Cryoelectron Microscopy
Biological Science Disciplines
Atomic Force Microscopy
life sciences
Fluorescence Microscopy
manipulation
Optical tweezers
methodology
Fluorescence microscopy
atomic force microscopy
Electron microscopy
Conformations
Atomic force microscopy
fluorescence microscopy
Research

Keywords

  • Atomic force microscopy
  • Biophysics
  • Electron microscopy
  • Fluorescence microscopy
  • Optical tweezers
  • Single-molecule

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Social Sciences(all)

Cite this

Bujalowski, P., Sherman, M., & Oberhauser, A. (2013). Single-molecule methods. In Molecular Biophysics for the Life Sciences (pp. 257-288). Springer New York. https://doi.org/10.1007/978-1-4614-8548-3_8

Single-molecule methods. / Bujalowski, Pawel; Sherman, Michael; Oberhauser, Andres.

Molecular Biophysics for the Life Sciences. Springer New York, 2013. p. 257-288.

Research output: Chapter in Book/Report/Conference proceedingChapter

Bujalowski, P, Sherman, M & Oberhauser, A 2013, Single-molecule methods. in Molecular Biophysics for the Life Sciences. Springer New York, pp. 257-288. https://doi.org/10.1007/978-1-4614-8548-3_8
Bujalowski P, Sherman M, Oberhauser A. Single-molecule methods. In Molecular Biophysics for the Life Sciences. Springer New York. 2013. p. 257-288 https://doi.org/10.1007/978-1-4614-8548-3_8
Bujalowski, Pawel ; Sherman, Michael ; Oberhauser, Andres. / Single-molecule methods. Molecular Biophysics for the Life Sciences. Springer New York, 2013. pp. 257-288
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