Single-molecule methods

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Single-molecule methods have emerged as powerful tools in life science research. These techniques allow the detection and manipulation of individual biological molecules and investigate, with unprecedented resolution, their conformations and dynamics at the nanoscale level. These techniques overcome the restrictions of traditional bulk biochemical studies by focusing on individuals of molecules. Here we describe some of the most common single-molecule methods including atomic force microscopy, optical tweezers, and fluorescence microscopy. We also describe the use of cryo-electron microscopy methods to study large molecules and macromolecular assemblies. We outline the principles of operation for each technique and discuss prominent applications.

Original languageEnglish (US)
Title of host publicationMolecular Biophysics for the Life Sciences
PublisherSpringer New York
Number of pages32
ISBN (Electronic)9781461485483
ISBN (Print)9781461485476
StatePublished - Jan 1 2013


  • Atomic force microscopy
  • Biophysics
  • Electron microscopy
  • Fluorescence microscopy
  • Optical tweezers
  • Single-molecule

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Biochemistry, Genetics and Molecular Biology
  • General Social Sciences


Dive into the research topics of 'Single-molecule methods'. Together they form a unique fingerprint.

Cite this