Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth

Guoyang Luo; Thomas Morgan; Mert O. Bahtiyar; Victoria V. Snegovskikh; Frederick Schatz; Edward Kuczynski; Edmund F. Funai; Antonette T. Dulay; Se Te Joseph Huang; Catalin S. Buhimschi; Irina A. Buhimschi; Stephen J. Fortunato; Ramkumar Menon; Charles J. Lockwood; Errol R. Norwitz

doi:10.1177/1933719107310990

Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth

Guoyang Luo, Thomas Morgan, Mert O. Bahtiyar, Victoria V. Snegovskikh, Frederick Schatz, Edward Kuczynski, Edmund F. Funai, Antonette T. Dulay, Se Te Joseph Huang, Catalin S. Buhimschi, Irina A. Buhimschi, Stephen J. Fortunato, Ramkumar Menon, Charles J. Lockwood, Errol R. Norwitz

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by χ² test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m² (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P =.02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.

Original language	English (US)
Pages (from-to)	147-155
Number of pages	9
Journal	Reproductive Sciences
Volume	15
Issue number	2
DOIs	https://doi.org/10.1177/1933719107310990
State	Published - Feb 2008
Externally published	Yes

Keywords

Human pregnancy
Preterm birth
Progesterone receptor
Progresterone
Single nucleotide polymorphisms

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1177/1933719107310990

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Luo, G., Morgan, T., Bahtiyar, M. O., Snegovskikh, V. V., Schatz, F., Kuczynski, E., Funai, E. F., Dulay, A. T., Huang, S. T. J., Buhimschi, C. S., Buhimschi, I. A., Fortunato, S. J., Menon, R., Lockwood, C. J., & Norwitz, E. R. (2008). Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth. Reproductive Sciences, 15(2), 147-155. https://doi.org/10.1177/1933719107310990

Luo, G, Morgan, T, Bahtiyar, MO, Snegovskikh, VV, Schatz, F, Kuczynski, E, Funai, EF, Dulay, AT, Huang, STJ, Buhimschi, CS, Buhimschi, IA, Fortunato, SJ, Menon, R, Lockwood, CJ & Norwitz, ER 2008, 'Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth', Reproductive Sciences, vol. 15, no. 2, pp. 147-155. https://doi.org/10.1177/1933719107310990

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abstract = "Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by χ2 test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m2 (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P =.02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.",

keywords = "Human pregnancy, Preterm birth, Progesterone receptor, Progresterone, Single nucleotide polymorphisms",

author = "Guoyang Luo and Thomas Morgan and Bahtiyar, {Mert O.} and Snegovskikh, {Victoria V.} and Frederick Schatz and Edward Kuczynski and Funai, {Edmund F.} and Dulay, {Antonette T.} and Huang, {Se Te Joseph} and Buhimschi, {Catalin S.} and Buhimschi, {Irina A.} and Fortunato, {Stephen J.} and Ramkumar Menon and Lockwood, {Charles J.} and Norwitz, {Errol R.}",

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AU - Luo, Guoyang

AU - Morgan, Thomas

AU - Bahtiyar, Mert O.

AU - Snegovskikh, Victoria V.

AU - Schatz, Frederick

AU - Kuczynski, Edward

AU - Funai, Edmund F.

AU - Dulay, Antonette T.

AU - Huang, Se Te Joseph

AU - Buhimschi, Catalin S.

AU - Buhimschi, Irina A.

AU - Fortunato, Stephen J.

AU - Menon, Ramkumar

AU - Lockwood, Charles J.

AU - Norwitz, Errol R.

PY - 2008/2

Y1 - 2008/2

N2 - Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by χ2 test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m2 (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P =.02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.

AB - Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by χ2 test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m2 (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P =.02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.

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KW - Progesterone receptor

KW - Progresterone

KW - Single nucleotide polymorphisms

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Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth

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