Sir2 Deacetylates Histone H3 Lysine 56 to Regulate Telomeric Heterochromatin Structure in Yeast

Feng Xu, Qiongyi Zhang, Kangling Zhang, Wei Xie, Michael Grunstein

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

At telomeric heterochromatin in yeast, the Sir protein complex spreads from Rap1 sites to silence adjacent genes. This cascade is believed to occur when Sir2, an NAD+-dependent enzyme, deacetylates histone H3 and H4 N termini, in particular histone H4 K16, enabling more Sir protein binding. Lysine 56 of histone H3 is located at the entry-exit points of the DNA superhelix surrounding the nucleosome, where it may control DNA compaction. We have found that K56 substitutions disrupt silencing severely without decreasing Sir protein binding at the telomere. Our in vitro and in vivo data indicate that Sir2 deacetylates K56 directly in telomeric heterochromatin to compact chromatin and prevent access to RNA polymerase and ectopic bacterial dam methylase. Since the spread of Sir proteins is necessary but not sufficient for silencing, we propose that silencing occurs when Sir2 deacetylates H3 K56 to close the nucleosomal entry-exit gates, enabling compaction of heterochromatin.

Original languageEnglish (US)
Pages (from-to)890-900
Number of pages11
JournalMolecular Cell
Volume27
Issue number6
DOIs
StatePublished - Sep 21 2007
Externally publishedYes

Fingerprint

Heterochromatin
Histones
Lysine
Yeasts
Fungal Proteins
Nucleosomes
DNA
Telomere
DNA-Directed RNA Polymerases
Protein Binding
NAD
Chromatin
Proteins
Enzymes
Genes

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Sir2 Deacetylates Histone H3 Lysine 56 to Regulate Telomeric Heterochromatin Structure in Yeast. / Xu, Feng; Zhang, Qiongyi; Zhang, Kangling; Xie, Wei; Grunstein, Michael.

In: Molecular Cell, Vol. 27, No. 6, 21.09.2007, p. 890-900.

Research output: Contribution to journalArticle

Xu, Feng ; Zhang, Qiongyi ; Zhang, Kangling ; Xie, Wei ; Grunstein, Michael. / Sir2 Deacetylates Histone H3 Lysine 56 to Regulate Telomeric Heterochromatin Structure in Yeast. In: Molecular Cell. 2007 ; Vol. 27, No. 6. pp. 890-900.
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