Sirtuins 1 and 2 Are Universal Histone Deacetylases

Willie Hsu, Bo Wu, Wenshe R. Liu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Lysine acetylation serves as an epigenetic marker for myriad cellular processes, such as signaling, differentiation, DNA repair, angiogenesis, and the like. Sirtuin 1 (SIRT1) and sirtuin 2 (SIRT2) are NAD+-dependent histone deacetylases that operate as post-translational regulators for the deacetylation of acetyllysine. Here, we discuss the ability for SIRT1 and SIRT2 to deacetylate monoacetylated histone H3 on two separate architectures - the peptide and the nucleosome. In addition, we analyze the site-specificity of SIRT1 and SIRT2 on 10 different monoacetylated histone H3 nucleosomes. By utilizing a rapid screening array, SIRT1 and SIRT2 were found to demonstrate heightened enzymatic activity when incubated with nucleosomal substrates over their peptide counterparts. These two enzymes displayed little site-specificity among the acetyl-nucleosomes screened, contrary to previous expectations, as well. The implication of the overall nonspecificity of SIRT1 and SIRT2 on the nucleosome suggests that these sirtuin enzymes have an adaptive nature, harnessing an ability to respond to various cellular situations, rather than an enzyme specifically designed for a particular task or function.

Original languageEnglish (US)
Pages (from-to)792-799
Number of pages8
JournalACS Chemical Biology
Volume11
Issue number3
DOIs
StatePublished - Mar 18 2016
Externally publishedYes

Fingerprint

Sirtuin 2
Sirtuin 1
Sirtuins
Histone Deacetylases
Nucleosomes
Histones
Group III Histone Deacetylases
Enzymes
Acetylation
Peptides
Epigenomics
DNA Repair
Lysine
Screening
Repair
DNA
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

Sirtuins 1 and 2 Are Universal Histone Deacetylases. / Hsu, Willie; Wu, Bo; Liu, Wenshe R.

In: ACS Chemical Biology, Vol. 11, No. 3, 18.03.2016, p. 792-799.

Research output: Contribution to journalArticle

Hsu, Willie ; Wu, Bo ; Liu, Wenshe R. / Sirtuins 1 and 2 Are Universal Histone Deacetylases. In: ACS Chemical Biology. 2016 ; Vol. 11, No. 3. pp. 792-799.
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