Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

Thomas A. Hardy, David J. Baker, Edward M. Newman, Lawrence C. Sowers, Myron F. Goodman, Steven S. Smith

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number1
DOIs
StatePublished - May 29 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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