Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

Thomas A. Hardy; David J. Baker; Edward M. Newman; Lawrence C. Sowers; Myron F. Goodman; Steven S. Smith

doi:10.1016/0006-291X(87)91299-X

Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

Thomas A. Hardy, David J. Baker, Edward M. Newman, Lawrence C. Sowers, Myron F. Goodman, Steven S. Smith

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.

Original language	English (US)
Pages (from-to)	146-152
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(87)91299-X
State	Published - May 29 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase",

abstract = "M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.",

author = "Hardy, {Thomas A.} and Baker, {David J.} and Newman, {Edward M.} and Sowers, {Lawrence C.} and Goodman, {Myron F.} and Smith, {Steven S.}",

note = "Funding Information: ACKNOWLEDGEMENTS: This work was supported primarily by grants from the Council for Tobacco Research (1571A), and the National Institutes of Health (GM-328631, with additional support from GM-21422, and GM-33863. S.S.S. and E.M.N. are members of the City of Hope Cancer Center CA-335172.",

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doi = "10.1016/0006-291X(87)91299-X",

language = "English (US)",

volume = "145",

pages = "146--152",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Elsevier B.V.",

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T1 - Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

AU - Hardy, Thomas A.

AU - Baker, David J.

AU - Newman, Edward M.

AU - Sowers, Lawrence C.

AU - Goodman, Myron F.

AU - Smith, Steven S.

N1 - Funding Information: ACKNOWLEDGEMENTS: This work was supported primarily by grants from the Council for Tobacco Research (1571A), and the National Institutes of Health (GM-328631, with additional support from GM-21422, and GM-33863. S.S.S. and E.M.N. are members of the City of Hope Cancer Center CA-335172.

PY - 1987/5/29

Y1 - 1987/5/29

N2 - M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.

AB - M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.

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Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

Abstract

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