Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase

Thomas A. Hardy, David J. Baker, Edward M. Newman, Lawrence Sowers, Myron F. Goodman, Steven S. Smith

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

M13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA(cytosine-5)methyltransferase. Rate enhancements of up to 35 fold are obtained depending on the size of the moiety at C-5. The enzyme appears optimally suited to sense a methyl group in one strand at this position. Alkaline density gradient analyses of the distribution of methyl groups applied to 5-BrdCyd or 5-IdCyd substituted DNA reveal that these groups serve to direct the enzyme to methylate the unsubstitued strand.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number1
DOIs
StatePublished - May 29 1987
Externally publishedYes

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DNA (Cytosine-5-)-Methyltransferase
Cytosine
Human Activities
Carbon
Deoxycytidine
DNA
Enzymes
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase. / Hardy, Thomas A.; Baker, David J.; Newman, Edward M.; Sowers, Lawrence; Goodman, Myron F.; Smith, Steven S.

In: Biochemical and Biophysical Research Communications, Vol. 145, No. 1, 29.05.1987, p. 146-152.

Research output: Contribution to journalArticle

Hardy, Thomas A. ; Baker, David J. ; Newman, Edward M. ; Sowers, Lawrence ; Goodman, Myron F. ; Smith, Steven S. / Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA(cytosine-5)methyltransferase. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 145, No. 1. pp. 146-152.
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