SK HEP-1

A human cell line of endothelial origin

Sue C. Heffelfinger, Hal H. Hawkins, Jim Barrish, Linda Taylor, Gretchen J. Darlington

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

SK-HEP-1 is an immortal, human cell line derived from the ascitic fluid of a patient with adenocarcinoma of the liver. We have determined that these cells are of endothelial origin. Despite the location of the tumor from which SK HEP-1 was derived, the cell line does not have properties of hepatocytes. Northern blot analysis of total cellular RNA shows no messenger RNA for the hepatic-specific proteins albumin, alpha-fibrinogen, or gamma-fibrinogen. Endothelial characteristics are seen by transmission electron microscopy. These features include numerous pinocytotic vesicles, electron dense granules consistent with Weibel-Palade bodies, and abundant intermediate filaments, identified immunocytochemically as vimentin. Cultures grown on plastic dishes grow in bundles of polygonal to spindle-shaped cells. Proteins characteristic for endothelial cells are identified by immunocytochemistry. Addition of basement membrane material (Matrigel) or type I collagen to the cultures induces these cells to organize into a tubular network.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume28
Issue number2
DOIs
StatePublished - Feb 1992
Externally publishedYes

Fingerprint

Fibrinogen
Endothelial Cells
Weibel-Palade Bodies
Cells
Cell Line
Intermediate Filaments
Ascitic Fluid
Liver
Endothelial cells
Vimentin
Collagen Type I
Transmission Electron Microscopy
Basement Membrane
Northern Blotting
Plastics
Tumors
Hepatocytes
Proteins
Adenocarcinoma
Cell Culture Techniques

Keywords

  • endothelium
  • Matrigel
  • SK HEP-1
  • tubule

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Heffelfinger, S. C., Hawkins, H. H., Barrish, J., Taylor, L., & Darlington, G. J. (1992). SK HEP-1: A human cell line of endothelial origin. In Vitro Cellular & Developmental Biology - Animal, 28(2), 136-142. https://doi.org/10.1007/BF02631017

SK HEP-1 : A human cell line of endothelial origin. / Heffelfinger, Sue C.; Hawkins, Hal H.; Barrish, Jim; Taylor, Linda; Darlington, Gretchen J.

In: In Vitro Cellular & Developmental Biology - Animal, Vol. 28, No. 2, 02.1992, p. 136-142.

Research output: Contribution to journalArticle

Heffelfinger, SC, Hawkins, HH, Barrish, J, Taylor, L & Darlington, GJ 1992, 'SK HEP-1: A human cell line of endothelial origin', In Vitro Cellular & Developmental Biology - Animal, vol. 28, no. 2, pp. 136-142. https://doi.org/10.1007/BF02631017
Heffelfinger SC, Hawkins HH, Barrish J, Taylor L, Darlington GJ. SK HEP-1: A human cell line of endothelial origin. In Vitro Cellular & Developmental Biology - Animal. 1992 Feb;28(2):136-142. https://doi.org/10.1007/BF02631017
Heffelfinger, Sue C. ; Hawkins, Hal H. ; Barrish, Jim ; Taylor, Linda ; Darlington, Gretchen J. / SK HEP-1 : A human cell line of endothelial origin. In: In Vitro Cellular & Developmental Biology - Animal. 1992 ; Vol. 28, No. 2. pp. 136-142.
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