Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise

Micah J. Drummond, Christopher Fry, Erin L. Glynn, Kyle L. Timmerman, Jared M. Dickinson, Dillon K. Walker, David M. Gundermann, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Amino acid transporters and mammalian target of rapamycin complex 1 (mTORC1) signaling are important contributors to muscle protein anabolism. Aging is associated with reduced mTORC1 signaling following resistance exercise, but the role of amino acid transporters is unknown. Young (n = 13; 28 ± 2 yr) and older (n = 13; 68 ± 2 yr) subjects performed a bout of resistance exercise. Skeletal muscle biopsies (vastus lateralis) were obtained at basal and 3, 6, and 24 h postexercise and were analyzed for amino acid transporter mRNA and protein expression and regulators of amino acid transporter transcription utilizing realtime PCR and Western blotting. We found that basal amino acid transporter expression was similar in young and older adults (P > 0.05). Exercise increased L-type amino acid transporter 1/solutelinked carrier (SLC) 7A5, CD98/SLC3A2, sodium-coupled neutral amino acid transporter 2/SLC38A2, proton-assisted amino acid transporter 1/SLC36A1, and cationic amino acid transporter 1/SLC7A1 mRNA expression in both young and older adults (P < 0.05). L-type amino acid transporter 1 and CD98 protein increased only in younger adults (P < 0.05). eukaryotic initiation factor 2 α-subunit (S52) increased similarly in young and older adults postexercise (P < 0.05). Ribosomal protein S6 (S240/244) and activating transcription factor 4 nuclear protein expression tended to be higher in the young, while nuclear signal transducer and activator of transcription 3 (STAT3) (Y705) was higher in the older subjects postexercise (P < 0.05). These results suggest that the rapid upregulation of amino acid transporter expression following resistance exercise may be regulated differently between the age groups, but involves a combination of mTORC1, activating transcription factor 4, eukaryotic initiation factor 2 α-subunit, and STAT3. We propose an increase in amino acid transporter expression may contribute to enhanced amino acid sensitivity following exercise in young and older adults. In older adults, the increased nuclear STAT3 phosphorylation may be indicative of an exercise-induced stress response, perhaps to export amino acids from muscle cells.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalJournal of Applied Physiology
Volume111
Issue number1
DOIs
StatePublished - Jul 2011

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Amino Acid Transport Systems
Young Adult
Skeletal Muscle
Exercise
STAT3 Transcription Factor
Activating Transcription Factor 4
Eukaryotic Initiation Factor-2
Cationic Amino Acid Transporter 1
Neutral Amino Acid Transport Systems
Ribosomal Protein S6
Amino Acids
Messenger RNA
Muscle Proteins
Quadriceps Muscle
Nuclear Proteins
Muscle Cells
Protons
Proteins
Up-Regulation
Age Groups

Keywords

  • Activating transcription factor 4
  • L-type amino acid transporter 1
  • Mammalian target of rapamycin complex 1
  • Proton-assisted amino acid transporter 1
  • Sarcopenia

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. / Drummond, Micah J.; Fry, Christopher; Glynn, Erin L.; Timmerman, Kyle L.; Dickinson, Jared M.; Walker, Dillon K.; Gundermann, David M.; Volpi, Elena; Rasmussen, Blake.

In: Journal of Applied Physiology, Vol. 111, No. 1, 07.2011, p. 135-142.

Research output: Contribution to journalArticle

Drummond, Micah J. ; Fry, Christopher ; Glynn, Erin L. ; Timmerman, Kyle L. ; Dickinson, Jared M. ; Walker, Dillon K. ; Gundermann, David M. ; Volpi, Elena ; Rasmussen, Blake. / Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. In: Journal of Applied Physiology. 2011 ; Vol. 111, No. 1. pp. 135-142.
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