Skeletal muscle autophagy and protein breakdown following resistance exercise are similar in younger and older adults

Christopher Fry, Micah J. Drummond, Erin L. Glynn, Jared M. Dickinson, David M. Gundermann, Kyle L. Timmerman, Dillon K. Walker, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Background.The loss of skeletal muscle mass and strength during aging, sarcopenia, increases the risk for falls and dependency. Resistance exercise (RE) training is effective at improving muscle mass and strength in older adults; however, aging is associated with reduced training-induced hypertrophy. Recent research has illustrated an impaired muscle protein synthetic response following an acute bout of RE in older adults but much less is known regarding the effect of acute RE on muscle protein breakdown (MPB). We hypothesize that the ubiquitin proteasome system and the autophagosomal-lysosomal system may regulate the overall rate of MPB during postexercise recovery.Methods.Muscle biopsies of the vastus lateralis were sampled from 16 older (age = 70±2 years) and 16 younger (age = 27±2 years) participants at baseline and at 3, 6, and 24 hours following an acute bout of RE. In conjunction with stable isotopic techniques to measure MPB, we utilized immunoblotting and RT-PCR to examine protein and mRNA expression for key signaling molecules in both the ubiquitin proteasome system and the autophagosomal-lysosomal system.Results.MuRF1 mRNA expression increased, whereas GABARAP mRNA decreased after RE in both younger and older adults (p <. 05). The LC3B-II/LC3B-I protein ratio decreased in both groups after RE (p <. 05), but MPB was not different 24 hour post-RE in either group (p >. 05).Conclusions.Aging does not influence skeletal MPB, autophagy, or the ubiquitin proteasome system following an acute bout of RE. Therefore, targeting the muscle protein synthesis response to exercise may hold more promise in the prevention of sarcopenia.

Original languageEnglish (US)
Pages (from-to)599-607
Number of pages9
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume68
Issue number5
DOIs
StatePublished - May 2013

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Muscle Proteins
Autophagy
Young Adult
Skeletal Muscle
Exercise
Proteasome Endopeptidase Complex
Ubiquitin
Sarcopenia
Muscle Strength
Messenger RNA
Resistance Training
Quadriceps Muscle
Immunoblotting
Hypertrophy
Biopsy
Muscles
Polymerase Chain Reaction
Research

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology
  • Medicine(all)

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Skeletal muscle autophagy and protein breakdown following resistance exercise are similar in younger and older adults. / Fry, Christopher; Drummond, Micah J.; Glynn, Erin L.; Dickinson, Jared M.; Gundermann, David M.; Timmerman, Kyle L.; Walker, Dillon K.; Volpi, Elena; Rasmussen, Blake.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 68, No. 5, 05.2013, p. 599-607.

Research output: Contribution to journalArticle

Fry, Christopher ; Drummond, Micah J. ; Glynn, Erin L. ; Dickinson, Jared M. ; Gundermann, David M. ; Timmerman, Kyle L. ; Walker, Dillon K. ; Volpi, Elena ; Rasmussen, Blake. / Skeletal muscle autophagy and protein breakdown following resistance exercise are similar in younger and older adults. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2013 ; Vol. 68, No. 5. pp. 599-607.
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abstract = "Background.The loss of skeletal muscle mass and strength during aging, sarcopenia, increases the risk for falls and dependency. Resistance exercise (RE) training is effective at improving muscle mass and strength in older adults; however, aging is associated with reduced training-induced hypertrophy. Recent research has illustrated an impaired muscle protein synthetic response following an acute bout of RE in older adults but much less is known regarding the effect of acute RE on muscle protein breakdown (MPB). We hypothesize that the ubiquitin proteasome system and the autophagosomal-lysosomal system may regulate the overall rate of MPB during postexercise recovery.Methods.Muscle biopsies of the vastus lateralis were sampled from 16 older (age = 70±2 years) and 16 younger (age = 27±2 years) participants at baseline and at 3, 6, and 24 hours following an acute bout of RE. In conjunction with stable isotopic techniques to measure MPB, we utilized immunoblotting and RT-PCR to examine protein and mRNA expression for key signaling molecules in both the ubiquitin proteasome system and the autophagosomal-lysosomal system.Results.MuRF1 mRNA expression increased, whereas GABARAP mRNA decreased after RE in both younger and older adults (p <. 05). The LC3B-II/LC3B-I protein ratio decreased in both groups after RE (p <. 05), but MPB was not different 24 hour post-RE in either group (p >. 05).Conclusions.Aging does not influence skeletal MPB, autophagy, or the ubiquitin proteasome system following an acute bout of RE. Therefore, targeting the muscle protein synthesis response to exercise may hold more promise in the prevention of sarcopenia.",
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AU - Timmerman, Kyle L.

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AU - Rasmussen, Blake

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